IP02
Protein A Agarose Suspension
Protein A Agarose Suspension designed for immunoprecipitation applications
제품 이름
Protein A Agarose Suspension, Protein A Agarose Suspension designed for immunoprecipitation applications
form
slurry (Liquid)
contains
≤0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
shipped in
wet ice
storage temp.
2-8°C
Disclaimer
Toxicity: Standard Handling (A)
General description
Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for mouse IgG2a and IgG2b and rabbit IgG.
Protein A conjugated to agarose.
Other Notes
Agarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG2a or IgG2b monoclonal antibodies. Mouse IgG1 or IgG3 and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Physical form
In PBS.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Derek W Stouth et al.
American journal of physiology. Cell physiology, 314(2), C177-C190 (2017-11-03)
Protein arginine methyltransferase 1 (PRMT1), PRMT4, and PRMT5 catalyze the methylation of arginine residues on target proteins. Previous work suggests that these enzymes regulate skeletal muscle plasticity. However, the function of PRMTs during disuse-induced muscle remodeling is unknown. The purpose
Maria-Eleni Lalioti et al.
Journal of cell science, 132(11) (2019-04-28)
A distinct combination of transcription factors elicits the acquisition of a specific fate and the initiation of a differentiation program. Multiciliated cells (MCCs) are a specialized type of epithelial cells that possess dozens of motile cilia on their apical surface.
Jonah Beenstock et al.
Molecular and cellular biology, 36(10), 1540-1554 (2016-03-16)
Many enzymes are self-regulated and can either inhibit or enhance their own catalytic activity. Enzymes that do both are extremely rare. Many protein kinases autoactivate by autophosphorylating specific sites at their activation loop and are inactivated by phosphatases. Although mitogen-activated
Ajay Bhargava et al.
eLife, 5 (2016-02-18)
The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010
Lanlan Wei et al.
Biochemical and biophysical research communications, 368(2), 279-284 (2008-01-29)
The recruitment of the bromodomains of CREB-binding protein (CBP) and p300 by the acetylated myogenic transcription factor MyoD was previously shown to be critical for the enhanced MyoD transcriptional activity following acetylation at its Lys99 and Lys102 positions. However, the
관련 콘텐츠
We manufacture IP and antibody purification agarose beads that purify immunoglobulins and IgG fractions. Our kits also provide a total antibody purification process
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| IP02-1.5MLCN | 04055977220209 |
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