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Merck

PEZGS0416

Millicell® EZ Slide

4-well glass chamber slide, 1.7 cm2/well, 16/pk, sterile

동의어(들):

4 well glass slide, Chamber slides

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32011202
Material:
polypropylene wells, polystyrene lid, soda-lime glass slide
Sterility:
ethylene oxide treated, sterile
Packaging:
box of 16 (4 trays, 4 units/tray)
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Quality Level

material

polypropylene wells, polystyrene lid, soda-lime glass slide

sterility

ethylene oxide treated, sterile

feature

Raised corner ridges on the lid facilitate stacking of multiple units

packaging

box of 16 (4 trays, 4 units/tray)

manufacturer/tradename

Millicell®

technique(s)

cell based assay: suitable, cell culture | mammalian: suitable, immunocytochemistry: suitable

L × W × H

75 mm × 25 mm × 1 mm, slide only, 77.8 mm × 33.8 mm × 13.9, excluding lid

L × W

77.8 mm × 33.8 mm × 16.2 mm, including lid

size

4 wells

well surface area

1.7 cm2

well volume

500-1700 μL

shipped in

ambient

storage temp.

10-30°C

General description

Millicell® EZ Slide allows the user to grow, fix, and stain cells directly on a microscope slide. There is no need to disassemble the unit or transfer cells from one device to another prior to analysis. The polypropylene wells are resistant to most fixatives used, so the cells can be grown on the slide and stained right in the well. Once stained, the microscope slide can be easily removed by pressing down the four side tabs of the unit and lifting the wells off of the slide. This easy slide removal reduces the risk of damaging the cells and no special tools are required to remove the wells from the slide. Raised corner ridges on the cover facilitate stacking of multiple units in the incubator, or they can be incubated in the tray supplied with the units.

Preparation Note

  1. Culture, fix, and stain the cells according to preferred protocol.
  2. To access the microscope slide, hold the Millicell® EZ SLIDE down on a flat surface and break the four tabs by pushing them down with the thumb. Remove the cover. Hold the slide down and lift the wells upward to remove. Remove slide from holder.

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany


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관련 콘텐츠

Expression of genetically-encoded fluorescently-tagged proteins has widely been employed for real-time visualization of cellular behavior and trafficking. Prepackaged, ready-to-use, high-titer lentiviral particles (which we have termed “lentiviral biosensors”) encoding GFP- or RFP-tagged proteins are a convenient, robust solution for fluorescent imaging of transduced cells. Compared to other nonviral transfection methods, lentiviral transduction, in many cases, offers higher transfection efficiency and more homogeneous protein expression, particularly for traditionally hard-to-transfect primary cell types. Lentiviral biosensors are ideal for use with fixed and live cell fluorescent microscopy, and are non-disruptive towards cellular function. GFP- or RFP-protein localization matches well with antibody-based immunostaining and demonstrates altered patterns of expression upon treatment with modulators of cell function and phenotype. Lentiviral biosensors provide a broadly effective, convenient method for visualization of cell behavior under a variety of physiological and pathological treatment conditions, in both endpoint and real-time imaging modalities. In this study, we focus on lentiviral biosensors containing GFP-LC3 and RFP-LC3 for the study of autophagosome formation.


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SKUGTIN
PEZGS041604053252699948