Milli-Mark^® Anti-CD54-FITC Antibody, clone 6.5B5

Protein A purified

Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Control
Human Lymphocytes

Evaluated by flow cytometry using human lymphocytes

Milli-Mark Anti-CD54-FITC Antibody, clone 6.5B5 is an antibody against CD54 for use in FC.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Antibody recognizes Human CD54 antigen.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

90 kDa (Springer, 1990)

The CD54 antigen is a single chain glycoprotein with a molecular mass of 90 kDa (Springer, 1990). It is the human intercellular adhesion molecule-1 (ICAM-1) belonging to the immunoglobulin supergene family. The ligand for ICAM-1 is LFA-1 (leucocyte function associated-1 protein) (Berendt, 1992). ICAM-1 is widely expressed on many cell types, although the degree of expression by unstimulated resting cells varies. As a cellular activation antigen the expression of ICAM-1 is up-regulated upon cell activation, being induced particularly in response to cytokines such as interleukin-1, tumor necrosis factor, and interferon- (Berendt, 1992; Wellicome, 1990; Thornhill, 1990). ICAM-1 might be a receptor for rhinovirus (Staunton, 1989). In rejecting kidneys the antibody stains all infiltrating cells strongly as well as glomerulus epithelium, endothelium on capillaries, large vessels, and mesangium (Waugh, 1989).

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

TNF-activated human umbilical vein endothelial cells