제품 이름
Anti-Collagen Type IV Antibody, clone 24.12.8 (PHM-12), clone 24.12.8 (PHM-12), Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
clone
24.12.8 (PHM-12), monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Application
Detect Collagen Type IV using this Anti-Collagen Type IV Antibody, clone 24.12.8 (PHM-12) validated for use in IH, IH(P).
The antibody is suitable for use on both frozen and protease digested paraffin processed tissue.
SUGGESTED USAGE
Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:40 will be applicable to most commonly used systems.
Optimal working dilutions must be determined by the end user.
SUGGESTED USAGE
Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:40 will be applicable to most commonly used systems.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
MAB1430 provides a marker for an antigen known to be important in glomerular cell-matrix interactions, and should prove useful in research into the mechanisms involved in renal pathology. This antibody reacts with glomerular and tubular basement membranes in kidney as well as basal lamina of capillaries. It also labels mesangial cells and matrix within the glomerulus as well as basement membrane structures in all organs examined (Gusterson et al., 1984).
General description
BIOCHEMISTRY:
p I = 5.5
p I = 5.5
Immunogen
Human glomeruli
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified immunoglobulin presented as a liquid in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide.
Preparation Note
Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
저장 등급
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Fibroblasts facilitate re-epithelialization in wounded human skin equivalents.
El Ghalbzouri, Abdoelwaheb, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 84, 102-112 (2004)
Functional characterization of cancer-associated fibroblasts of human cutaneous squamous cell carcinoma.
Suzan Commandeur,Suet Huy Ho,Frank R de Gruijl,Rein Willemze,Cornelis P Tensen et al.
Experimental Dermatology null
Invading squamous cell carcinoma can retain a basal lamina. An immunohistochemical study using a monoclonal antibody to type IV collagen.
Gusterson, B A, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 51, 82-87 (1984)
Morphofunctional studies of the glomerular wall in mice lacking entactin-1.
Simon-Philippe Lebel, Yong Chen, Diane Gingras, Albert E Chung, Moise Bendayan
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society null
S E Aggrey et al.
Poultry science, 73(12), 1822-1828 (1994-12-01)
Records of 1,530 Japanese quail were used to estimate heritabilities and genetic correlations based on a derivative-free restricted maximum likelihood (REML) method with an animal model and ANOVA. The animal model included fixed effects of hatch and sex, random effects
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| MAB1430 | 04053252666605 |
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