MAB3242
Anti-Smoothelin Antibody, clone R4A
clone R4A, Chemicon®, from mouse
동의어(들):
Clone R4A Anti-Smoothelin, Smoothelin Detection Antibody
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
제품 이름
Anti-Smoothelin Antibody, clone R4A, clone R4A, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
clone
R4A, monoclonal
species reactivity
pig, chicken, monkey, canine, feline, human, hamster
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SMTN(6525)
Application
Detect Smoothelin using this Anti-Smoothelin Antibody, clone R4A validated for use in WB, IC, IH(P).
Western blot
Immunocytochemistry
Immunohistochemistry on both frozen and paraffin embedded tissue sections. For paraffin sections, high temperature antigen retrieval in 10 mM citrate buffer (pH 6.0) is highly recommended (van der Loop, 1997).
Optimal working dilutions must be determined by the end user.
Immunocytochemistry
Immunohistochemistry on both frozen and paraffin embedded tissue sections. For paraffin sections, high temperature antigen retrieval in 10 mM citrate buffer (pH 6.0) is highly recommended (van der Loop, 1997).
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
MAB3242 reacts with smoothelin, a cytoskeletal protein exclusively found in smooth muscle cells. Reacts with the 59 kDa and 100 kDa protein corresponding to smoothelin by Western blot.
General description
Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cell with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin or vimentin. Smoothelin colocalizes with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 50 kDa isoform specific for visceral SMC, and an isoform with a molecular weight of approximately 100 kDa in vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22.MAB3242 is useful to discriminate fully differentiated, contractile SMCs from proliferative SMCs. The distribution of smoothelin-positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Smoothelin is a useful tool in the evaluation of atherosclerotic lesions.
Immunogen
Cytoskeletal extract of chicken gizzard.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Liquid in buffer (PBS) with 0.1% sodium azide.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
저장 등급
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
H Ohta et al.
British journal of cancer, 100(2), 389-398 (2008-12-25)
We previously reported hedgehog (Hh) signal activation in the mucus-secreting pit cell of the stomach and in diffuse-type gastric cancer (GC). Epithelial-mesenchymal transition (EMT) is known to be involved in tumour malignancy. However, little is known about whether and how
Sachiko Matsuzaki et al.
Human reproduction (Oxford, England), 31(3), 541-553 (2016-01-15)
Can deep infiltrating endometriotic stromal cells (DES) sense changes in extracellular matrix (ECM) stiffness and respond to them? Soft matrices inhibit cell proliferation and inactivate the fibrotic phenotype of DES in vitro. Deep infiltrating endometriosis (DIE) is characterized histologically by
Survivin expression is up-regulated in vascular injury and identifies a distinct cellular phenotype.
Hector F Simosa, Grace Wang, XinXin Sui, Timothy Peterson, Vinod Narra, Dario C Altieri et al.
Journal of Vascular Surgery null
Kristin Dawson et al.
PloS one, 7(12), e52032-e52032 (2012-12-20)
Fibroblasts are important in the atrial fibrillation (AF) substrate resulting from congestive heart failure (CHF). We previously noted changes in in vivo indices of fibroblast function in a CHF dog model, but could not detect changes in isolated cells. This
Eric van der Veer et al.
Circulation research, 97(1), 25-34 (2005-06-11)
Conversion of vascular smooth muscle cells (SMCs) from a proliferative state to a nonproliferative, contractile state confers vasomotor function to developing and remodeling blood vessels. Using a maturation-competent human SMC line, we determined that this shift in phenotype was accompanied
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| MAB3242 | 04053252611476 |
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