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Merck

MABN844

Anti-Neurophysin 1 Antibody, clone PS 38

clone PS 38, from mouse

동의어(들):

Oxytocin-neurophysin 1, OT-NPI, Oxytocin, Ocytocin, Neurophysin 1

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
PS 38, monoclonal
Application:
immunohistochemistry
radioimmunoassay
western blot
Species reactivity:
rat
Citations:
10
Technique(s):
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: suitable
Uniprot accession no.:
P01179

주요 문서

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제품 이름

Anti-Neurophysin 1 Antibody, clone PS 38, clone PS 38, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PS 38, monoclonal

species reactivity

rat

technique(s)

immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_037128

UniProt accession no.

P01179

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

rat ... Oxt(25504)

Analysis Note

Evaluated by Western Blotting in rat pituitary tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Neurophysin 1 in 10 µg of rat pituitary tissue lysate.

Application

Immunohistochemistry: A representative lot detected oxytocin (OT)-synthesizing neurons in the hypothalamic supraoptic (SON) and neural lobe (NL) regions of paraffin-embedded rat hypothalamus and pituitary tissue sections (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Radioimmunoassay (RIA): A representative lot was assessed for binding affinity toward purified rat neurophysinn 1 by RIA (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Western Blotting Analysis: A representative lot detected neurophysinn 1 in rat posterior pituitary extracts (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
This Anti-Neurophysin 1 Antibody, clone PS 38 is validated for use in Western Blotting, Immunohistochemistry, Radioimmunoassay for the detection of Neurophysin 1.

Biochem/physiol Actions

Clone PS 38 detects neurophysin 1 (oxytocin-associated NP), but not ocytocin/oxytocin or neurophysin 2 (vasopressin-associated NP)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Neurophysins are linear cycteine-rich proteins containing 93-95 amino acids. Two types of neurophysins, NPI and NPII are found in most mammalian species and they serve as carriers of oxytocin (OT) and vasopressin (AVP), respectively. Each NP and its associated hormone is synthesized as one precursor protein, OT-NPI (UniProt P01179) encoded by the Oxt (or Ot) gene or VAP-NPII (UniProt P01186) encoded by the Avp gene in rat species. Post-translational cleavages remove the signal peptide and process the precursor into mature OT and NPI (aa 20-28 and aa 32-135, respectively, of OT-NPI), or AVP and NPII (aa 24-32 and aa 36-128, respectively, of AVP-NPII). OT is a potent hormone and neurotransmitter secreted within the pituitary gland. OT exerts its biological activity through its receptor OXTR (also known as OTR). OT is used clinically for labor induction and may exhibit therapeutic potential for treating social anxiety disorders. VAP functions primarily as an anti-diuretic and regulates the concentration of water, glucose, and salts in blood. VAP is secreted from the posterior pituitary, but exerts its biological activity in the kidney via the G protein-coupled receptor AVPR2 (V2R).
~12 kDa observed

Immunogen

Epitope: Neurophysin 1
KLH-conjugated acid soluble extract of rat (Sprague-Dawley) intermediate lobes corresponding to rat Neurophysin 1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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저장 등급

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

시험 성적서(COA)

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문서 라이브러리 방문

Leo Kojima et al.
iScience, 27(6), 109878-109878 (2024-05-27)
Adeno-associated virus (AAV) vectors are potential tools for cell-type-selective gene delivery to the central nervous system. Although cell-type-specific enhancers and promoters have been identified for AAV systems, there is limited information regarding the effects of AAV genomic components on the
Dipanwita Pati et al.
Journal of neuroendocrinology, 32(3), e12839-e12839 (2020-03-07)
Significant prior evidence indicates that centrally acting oxytocin robustly modulates stress responsiveness and anxiety-like behaviour, although the neural mechanisms behind these effects are not entirely understood. A plausible neural basis for oxytocin-mediated stress reduction is via inhibition of corticotrophin-releasing hormone
Rohan Savani et al.
Bioscience reports, 44(1) (2024-01-16)
Combining adeno-associated virus (AAV)-mediated expression of Cre recombinase with genetically modified floxed animals is a powerful approach for assaying the functional role of genes in regulating behavior and metabolism. Extensive research in diverse cell types and tissues using AAV-Cre has
Hanna Hörnberg et al.
Nature, 584(7820), 252-256 (2020-08-08)
A fundamental challenge in developing treatments for autism spectrum disorders is the heterogeneity of the condition. More than one hundred genetic mutations confer high risk for autism, with each individual mutation accounting for only a small fraction of cases1-3. Subsets
Marie Habart et al.
STAR protocols, 4(1), 101968-101968 (2023-01-05)
Here, we present an optimized iDISCO+ protocol combining tissue clearing and light sheet microscopy to map the postnatal development of oxytocin and vasopressin neurons in mouse hypothalamus. We describe tissue preparation, immunostaining, clearing, and imaging. We then detail how to
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White Paper: Further considerations of antibody validation and usage.

국제 무역 품목 번호

SKUGTIN
MABN84404055977181685

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