YUMMER1.7D4 Mouse Melanoma Cell Line

Mouse, Cancer Cells

This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

The YUMMER1.7D4 mouse melanoma cell line is both immunocompetent and reflective of the somatic mutations common in melanomas. YUMMER1.7D4 carries three driver mutations of melanoma: Braf V600E, Pten -/- and Cdkn2 -/- (1,2). In addition, the YUMMER1.7D4 cell line harbors a high frequency of stable UV-induced somatic mutations which have been shown to stimulate host adaptive immune response (3). YUMMER1.7D4 cells are diploid, allowing for enhanced knockout frequency in CRISPR-based screens. The unique features of the YUMMER1.7D4 cell line make it a valuable model for studies of immune checkpoint inhibition and mechanisms of anti-tumor responses.

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The YUMMER1.7D4 mouse melanoma cell line is a diploid clonal isolate from YUMM1.7D4 cells exposed to UVB radiation. The original YUMM1.7 cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57/B1/6 mouse into which mutations from the Braf/Pten genetically-engineered mouse model had been introduced via backcrossing (1). The YUMMER1.7D4 cell line harbors the Braf V600E mutation and is homozygous negative for wild-type Pten and Cdkn2 (3).

Research Category:
Cancer

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

&ge;1X10⁶ cells/vial

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.