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Merck

SCM304

3dGRO® Human Colon Organoid Expansion Medium

Complete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids.

동의어(들):

Colon Organoid Culture Media, Intestinal Organoid Culture Media

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.71
UNSPSC Code:
12352207

주요 문서

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기술 서비스
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도움 문의

form

liquid

packaging

pkg of 50 mL

technique(s)

cell culture | stem cell: suitable

osmolality

350-375 mOsm

application(s)

cell analysis

관련 카테고리

General description

3dGRO® Human Colon Organoid Expansion Medium is a complete ready-to-use serum-free medium for the expansion and long-term culture of human colonic organoids. The medium has been validated for use with 3dGRO® Human iPSC Derived Colon Organoids (Cat. No. SCC300). Colon organoids propagated in the medium express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B.

Application

Complete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids.

Research Category:
  • Cell Culture
  • Stem Cell Research

Preparation Note

Upon receipt, store at −20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly. Use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at −20°C until the expiry date.

Analysis Note

Appearance (Color): Clear/No Particlulates (Red Liquid)
Osmolality: 350-375 mOsm
pH: 7.0 – 7.4
Sterility Tested: No Growth/Pass
Endotoxin: <2 EU/mL
Mycoplasma: Negative
Functional Assay: Thaw and culture of human colon organoids for 2 passages.

Legal Information

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

저장 등급

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

3D Organoid Culture: New In Vitro Models of Development and Disease

Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

Using Human iPS Cell-derived Colon Organoids for Cytotoxicity Screening of Drug Compounds

Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.

Organoid Culture Frequently Asked Questions

Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.

오가노이드 배양 자주 묻는 질문

확립된 3차원 세포 배양 프로토콜에 따라 오가노이드를 배양하고 분석하는 방법을 배우세요. 팁과 요령.

프로토콜

Forskolin-Induced Swelling Assays using Millicell® Microwell Plates

Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.

In Vitro Differentiation of Human iPS Cells Into Colon Organoids in Serum-Free Cell Culture Conditions

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.

Forskolin-Induced Swelling Assays

A rapid in vitro assay for CFTR function, the forskolin-induced swelling protocol uses human colon organoids, which can be derived from cystic fibrosis patient tissue.

관련 콘텐츠

3D Cell Culture Workflow Tools

Explore our portfolio of 3D cell culture tools and technologies for any 3D cell line or application and discover organoids, hydrogels, ULA, microwell, or hydrogel plates, and more.

Culturing Colon Organoid Monolayers using Millicell® Hanging Inserts

Monitor barrier formation using colon PDOs, iPSC-derived colon organoids, Millicell® cell culture inserts, and the Millicell® ERS. 3.0.

3dGRO¿ Human Colon Organoid Expansion Medium

국제 무역 품목 번호

SKUGTIN
SCM30404054839986451

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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