form
liquid
Quality Level
contains
0.6-1.0% ethanol as stabilizer
suitability
suitable for nucleic acid purification
application(s)
agriculture
General description
Chloroform:Isoamyl Alcohol 24:1 is an organic reagent used for DNA extraction and precipitation.
Application
Chloroform:Isoamyl alcohol 24:1 has been used:
- to extract bacterial genomic DNA, DNA from Daphnia spp, and salmon sperms.
- for a single DNA precipitation step for high-quality DNA extraction.
- for protein extraction and RNAse treatment.
- in phenolic oxidation to separate phenolics from the aqueous portion.
- for RNA isolation from brown adipose tissue (BAT), inguinal white adipose tissue (iWAT), and epididymal adipose tissue (eWAT).
- in modified cetyltrimethyl ammonium bromide (CTAB) method to extract total RNA from Impatiens balsamina.
signalword
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3
target_organs
Central nervous system, Liver,Kidney
저장 등급
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
185.0 °F
flash_point_c
85 °C
관련 콘텐츠
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
세포, 조직, 혈액, 바이러스 및 기타 시료 유형으로부터 유전체 DNA, 플리스미드 DNA 및 총 RNA의 추출과 정제를 위한 일반적인 기법과 다운스트림 애플리케이션의 개요.
A modified CTAB-based protocol for total RNA extraction from the medicinal plant Impatiens balsamina (Balsaminaceae) for next-generation sequencing studies.
FOONG LC, et al.
Journal of Separation Science, 46(2) (2017)
Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species
Healey A, et al.
Plant methods, 10(1), 1-8 (2014)
A modified SDS-based DNA extraction method from raw soybean
Xia YC, et al.
Bioscience Reports, 39(2), BSR20182271-BSR20182271 (2019)