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Calcium Phosphate Transfection Kit

Name: Calcium Phosphate Transfection Kit
Brand: SIGMA

Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells

동의어(들):

Gene delivery

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:

12352200

EC Number:

233-140-8

NACRES:

NA.85

Form:

solution

Grade:

Molecular Biology

Technique(s):

transfection: suitable

기술 서비스

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도움 문의

속성

grade

Molecular Biology

Quality Level

200

form

solution

usage

kit sufficient for 160 transfections (6 cm dishes), kit sufficient for 400 transfections (3.5 cm dishes), kit sufficient for 80 transfections (10 cm dishes)

technique(s)

transfection: suitable

shipped in

dry ice

storage temp.

−20°C

설명

General description

Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types.

Application

Calcium Phosphate Transfection Kit has been used:

to enable transfection
to transfect Hek293T cells
to transfect H29D cells

Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method:

BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D

Biochem/physiol Actions

The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl₂ solution containing the DNA. A DNA-calcium phosphate co-precipitate forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.

Features and Benefits

Suitable for transient and stable transfection
Reproducible for a wide range of cell types
Widely referenced
Inexpensive

Other Notes

The Calcium Phosphate Transfection Kit contains:
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)

The protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.

pictograms

GHS07

signalword

Warning

hcodes

H319

pcodes

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

저장 등급

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 1

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Lot/Batch Number

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문서 라이브러리 방문

프로토콜

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

문서

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

세포 형질 도입 개론

전사는 유전 물질을 세포 내로 도입하여 유전자 발현 및 세포 생물학 연구를 지원합니다.