General description
Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Application
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Lambda DNA Mixed Digest has been used for DNA electrophoresis.
Preparation Note
Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.
Other Notes
For optimal resolution, the recommended agarose gel concentration is 0.4%.
Sigma′s Lambda DNA Mixed Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
저장 등급
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
문서
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
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