H7033
PerfectHyb™ Plus Hybridization Buffer
for Northern and Southern blotting, solution
동의어(들):
hyb buffer, hyb solution, hybridization solution, northern hybridization buffer, southern hybridization buffer
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
NACRES:
NA.25
UNSPSC Code:
12161700
General description
Sigma′s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours.
Application
Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots.
Features and Benefits
- Fast and easy-to-use
- Up to 20x more sensitive than conventional buffers
- Doesn′t require blocking steps
- Compatible with neutral and positively-charged membranes
- Compatible with chemiluminescent, colorimetric , and DIG detection assays
- Compatible with radioactive and non-radioactive probes
- Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes
Other Notes
A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion.
Legal Information
PerfectHyb is a trademark of Sigma-Aldrich Co. LLC
저장 등급
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
프로토콜
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
관련 콘텐츠
Instructions
Xianfeng Morgan Xu et al.
The Plant cell, 19(5), 1537-1548 (2007-05-22)
Vertebrate Tpr and its yeast homologs Mlp1/Mlp2, long coiled-coil proteins of nuclear pore inner basket filaments, are involved in mRNA export, telomere organization, spindle pole assembly, and unspliced RNA retention. We identified Arabidopsis thaliana NUCLEAR PORE ANCHOR (NUA) encoding a
Shinpei Kawaoka et al.
RNA (New York, N.Y.), 15(7), 1258-1264 (2009-05-23)
Genetic studies and large-scale sequencing experiments have revealed that the PIWI subfamily proteins and PIWI-interacting RNAs (piRNAs) play an important role in germ line development and transposon control. Biochemical studies in vitro have greatly contributed to the understanding of small
Edyta Koscianska et al.
TheScientificWorldJournal, 11, 102-117 (2011-01-11)
This protocol describes how to perform northern blot analyses to detect microRNAs and their precursors with single-nucleotide resolution, which is crucial for analyzing individual length variants and for evaluating relative quantities of unique microRNAs in cells. Northern blot analysis consists