form
lyophilized powder
specific activity
≥10 units/g
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
Application
Lysing Enzymes from Trichoderma harzianum is suitable retrieve DNA plugs from agarose gels. It has been used:
- for protoplast isolation
- for hydrolyzing poly(1-3)-glucose of the yeast cell wall glucan prior to yeast spheroplast transformation
- for the lysis of Aspergillus niger vegetative cells
Biochem/physiol Actions
Lysing Enzymes from Trichoderma harzianum is called Glucanex. It comprised of mixture of isoenzymes with β-1, 3 glucanase activity. Lysing Enzymes from Trichoderma harzianum hydrolyzes yeast cell wall oligosaccharides to glucan for protoplast preparation. Its stability is influenced by pH changes.
Analysis Note
Contains β-glucanase, cellulase, protease, and chitinase activities
Other Notes
One unit will hydrolyze casein to produce color equivalent to 1.0 micromole (181 μg) of tyrosine per minute at ph 7.5 at 37 deg C (color byfolin-ciocalteu reagent).
Legal Information
A product of Novozyme Corp.
Glucanex is a registered trademark of Novozymes Corp.
저장 등급
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Influence of protoplast fusion between two Trichoderma spp. on extracellular enzymes production and antagonistic activity
Hassan MM
Biotechnology, Biotechnological Equipment, 28(6), 1014-1023 (2014)
Divya Ramalingam Iyer et al.
PLoS genetics, 13(8), e1006958-e1006958 (2017-08-15)
In response to DNA damage during S phase, cells slow DNA replication. This slowing is orchestrated by the intra-S checkpoint and involves inhibition of origin firing and reduction of replication fork speed. Slowing of replication allows for tolerance of DNA
Identification of InuR, a new Zn (II) 2Cys6 transcriptional activator involved in the regulation of inulinolytic genes in Aspergillus niger
Yuan XL, et al.
Molecular Genetics and Genomics, 279(1), 11-26 (2008)