주요 문서

모든 문서 보기

P4707

Poly-ʟ-Lysine Hydrobromide

Name: Poly-ʟ-Lysine Hydrobromide
Brand: SIGMA

synthetic, liquid, 0.01%, suitable for cell culture

동의어(들):

PLL Solution

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

보기 변경

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:

25988-63-0

UNSPSC Code:

12352202

eCl@ss:

32160406

NACRES:

NA.75

MDL number:

MFCD00165424

Biological source:

synthetic

Form:

liquid

Technique(s):

cell culture | mammalian: suitable

Concentration:

0.01%

Mol wt:

70—150 kDa

기술 서비스

도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.

도움 문의

속성

제품 이름

Poly-L-lysine solution, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

biological source

synthetic

Quality Level

300

sterility

sterile-filtered

product line

BioReagent

form

liquid

mol wt

70—150 kDa

packaging

pkg of 50 mL

concentration

0.01%

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm²

impurities

endotoxin, tested

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

SMILES string

N([C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)C(=O)[C@@H](N)CCCCN

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

설명

General description

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Application

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Poly-L-lysine coated glass coverslips have been used for mounting samples for scanning electron microscopy.
Poly-L-lysine coated wells were used for trapping of PC-12 cells on electrodes.
Poly-L-lysine-coated borosilicate glass slides were used for the seeding of viral vector-transduced cells.

Biochem/physiol Actions

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Preparation Note

This product is supplied as a 0.01% sterile filtered solution in water. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.

Other Notes

Still not finding the right product?

Explore all of our products under

저장 등급

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

프로토콜

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

문서

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Poly-lysine Types for Biomedical Applications

Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.