콘텐츠로 건너뛰기
Merck

D7295

Deoxynucleotide Mix, 10 mM

Molecular Biology Reagent

동의어(들):

Deoxynucleotide Mix, 10 mM, 10mM dNTP mix, dNTP mix, dNTPs

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택


제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.52
UNSPSC Code:
41106305
Assay:
≥99%
Biological source:
synthetic (organic)
Form:
liquid
Solubility:
H2O: soluble at 20 °C
Storage temp.:
−20°C
기술 서비스
도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.
도움 문의
기술 서비스
도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.
도움 문의

biological source

synthetic (organic)

Quality Level

assay

≥99%

form

liquid

concentration

10 mM

color

colorless

solubility

H2O: soluble at 20 °C

application(s)

agriculture

foreign activity

DNase, RNase, none detected

shipped in

dry ice

storage temp.

−20°C

General description

Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.

Application

dNTP Mix has been used:

  • in the PCR amplification of genomic DNA isolated from insect, fungi, virus, human,
  • in reverse transcription of total RNA to cDNA.
  • as a component of the DNA amplification mixture for polymerase chain reaction (PCR)
  • routine and long PCR
  • manual and automated DNA sequencing
  • cDNA synthesis and labeling reactions

Features and Benefits

  • Purity of each dNTP: Minimum 99%
  • Conveniently formulated; 1 μL is used per 50 μL PCR
  • Equimolar amounts of each dNTP means less pipetting
  • Minimize risk of contamination in PCR
  • UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions

저장 등급

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Abril Sánchez-Botet et al.
Scientific reports, 8(1), 11797-11797 (2018-08-09)
Colorectal cancer (CRC) is one of the most common cancers worldwide, with 8-10% of these tumours presenting a BRAF (V600E) mutation. Cyclins are known oncogenes deregulated in many cancers, but the role of the new subfamily of atypical cyclins remains
Shangmei Cao et al.
Journal of ethnopharmacology, 244, 112104-112104 (2019-08-09)
ShenYanXiaoBai granules is a traditional Chinese herbal medicine, It is used widely for the treatment of proteinuria caused by various kidney diseases. This study investigated the mechanism of Shenyan Xiaobai Granule in the treatment of nephritis proteinuria. 100 male wistar
Unal Egeli et al.
Cancer investigation, 24(5), 484-491 (2006-08-31)
BRCA1 and BRCA2 gene mutations in patients with breast and/or ovarian cancer have been not characterized in the Turkish population until now. A total of 87 female subjects from two sets of families (38 families total) provided blood samples from
Billions and billions sold: Pet-feeder crickets (Orthoptera: Gryllidae), commercial cricket farms, an epizootic densovirus, and government regulations make for a potential disaster
Weissman, et al.
Zootaxa, 3504(1), 67-88 (2012)
Ajay V Maker et al.
Journal of the American College of Surgeons, 228(5), 721-729 (2019-02-23)
Current standard-of-care technologies, such as imaging and cyst fluid analysis, are unable to consistently distinguish intraductal papillary mucinous neoplasms (IPMNs) of the pancreas at high risk of pancreatic cancer from low-risk IPMNs. The objective was to create a single-platform assay

문서

SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.

프로토콜

표준 PCR 프로토콜 단계를 알아보고 시약 목록 또는 사이클링 매개변수를 검토하세요. 일상적인 DNA 증폭을 위한 이 방법은 표준 Taq DNA 중합효소를 사용합니다.

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

모두 보기

관련 콘텐츠

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.