form
liquid
usage
1 kit sufficient for 2,000 tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
technique(s)
protein quantification: suitable
application(s)
cell analysis
detection
detection method
colorimetric, fluorometric
storage temp.
2-8°C
Quality Level
General description
In Vitro Toxicology Assay Kit (Resazurin based) is designed for fluorometrically or spectrophotometrically determining cell number as a function of metabolic activity using the dye resazurin. The resazurin method is simple, accurate, and reproducible
Application
In Vitro Toxicology Assay Kit, Resazurin based has been used:
- in cell viability assay of human primary fibroblasts
- to determine the cell proliferation rate in murine pre-osteoblasts
- to determine the metabolic activity of microfluidic and monolayer cultures
Biochem/physiol Actions
Bioreduction of the dye reduces the amount of its oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).
저장 등급
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
U J Strotmann et al.
Ecotoxicology and environmental safety, 25(1), 79-89 (1993-02-01)
The dehydrogenase assay with resazurin as an oxidoreduction dye was used for monitoring the performance of six laboratory-scale sewage treatment plants. The dehydrogenase activity measured proved to be a reliable monitoring parameter, although it did not always positively correlate with
Comparison of several microbiological toxicity screening tests.
Dutka, B.J., et al.
Water Research, 17, 1363-1367 (1983)
A rapid biochemical test for measuring chemical toxicity.
D Liu
Bulletin of environmental contamination and toxicology, 26(2), 145-149 (1981-02-01)
Monika Marędziak et al.
Stem cells international, 2016, 2152435-2152435 (2016-03-05)
Tissue regeneration using human adipose derived mesenchymal stem cells (hASCs) has significant potential as a novel treatment for many degenerative bone and joint diseases. Previous studies have established that age negatively affects the proliferation status and the osteogenic and chondrogenic
A comparative study of methods for assessing the toxicity to bacteria of single chemicals and mixtures.
King, E.F. et al.
Toxicity Screening Procedures Using Bacterial Systems, 175-194 (1984)
문서
암, 신경과학 및 줄기세포 연구에 응용할 수 있는 세포 증식(BrdU, MTT, WST1), 세포 생존력 및 세포 독성 실험을 위한 세포 기반 분석입니다.
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.
관련 콘텐츠
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