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Merck

F2456

Fluorescein (free acid)

Dye content 95%, Powder

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About This Item

Fórmula empírica (notación de Hill):
C20H12O5
Número CAS:
2321-07-5
Peso molecular:
332.31
NACRES:
NA.47
PubChem Substance ID:
24894822
UNSPSC Code:
12171500
Colour Index Number:
45350:1
EC Number:
219-031-8
MDL number:
MFCD00005050
Beilstein/REAXYS Number:
94324

Nombre del producto

Fluorescein (free acid), Dye content 95 %

InChI

1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H

InChI key

GNBHRKFJIUUOQI-UHFFFAOYSA-N

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1

form

powder

composition

Dye content, 95%

technique(s)

titration: suitable

color

orange to red-brown

mp

320 °C (lit.)

λmax

496 nm

ε (extinction coefficient)

≥12000 at 282-286 nm
≥39000 at 236-240 nm
≥70000 at 488-492 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Fluorescein (free acid) has been used as a staining agent in retinal imaging.
Fluorescein (free acid) is widely used as a fluorescent stain and trace for many living cells and tissue applications. It is also suitable as a marker for the study of motility of bile canaliculi. Some of the clinical applications are evaluating the ocular surface and delineation of brain regions lacking a blood-brain barrier to permit accurate surgical resection of malignant glioma. Fluorescein angiography helps investigate changes in retinal blood supply. Fluorescein also plays a role as a physiological tracer to characterize the salmon′s intestinal permeability (Schep et al., 1998), and for the fluorophotometric measurement of aqueous humor flow. It is widely employed as a quantum yield standard.

General description

Fluorescein (free acid, CI 45350) is a xanthene dye. Fluorescein (free acid) molecule can be divided into two parts. One part contains xanthene moiety which acts as fluorophore and the other part of the molecule bears benzene moiety which is the fluorescence-controlling moiety.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000277631
0000277631
SHBP5589
MKCJ1475
10102PE

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