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Merck

AB5731

Anti-Nanog Antibody, NT

Chemicon®, from rabbit

Sinónimos:

Homeobox transcription factor Nanog, Nanog homeobox

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Nombre del producto

Anti-Nanog Antibody, NT, Chemicon®, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat, mouse

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

isotype

IgG

NCBI accession no.

NM_024865.2

UniProt accession no.

Q9H9S0

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... NANOG(79923)
mouse ... Nanog(71950)

Categorías relacionadas

General description

39 kDa (MW 34 kDa)
NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency, i.e., these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
NANOG may be useful in the immunohistochemical diagnosis of tumors. NANOG is expressed in germ cells of the fetus and in some germ cell tumors of the gonads and central nervous system (CNS).

Analysis Note

Control
Whole cell lysate from mouse embryonic stem cells.
Routinely evaluated by Western Blot on Embryonic Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected NANOG on 10 μg of Embryonic Mouse Brain lysates

Application

Detect Nanog using this Anti-Nanog Antibody, N-terminus validated for use in WB.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Western blotting:
0.5-1.0 µg/mL. The antibody recognizes a protein of ~35 kDa on NT2/D1 cell lysate. Additional bands of ~55 and ~130 kDa may be seen depending on sample and antibody dilution used.Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Antibody recognizes Nanog.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: N-terminus
Synthetic peptide from mouse Nanog.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified
Purified rabbit polyclonal immunoglobulin in buffer containing liquid 0.1% sodium azide.

Preparation Note

Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Establishing clonal cell lines with endothelial-like potential from CD9(hi), SSEA-1(-) cells in embryonic stem cell-derived embryoid bodies.
Lian, Q; Yeo, KS; Que, J; Tan, EK; Yu, F; Yin, Y; Salto-Tellez, M; Menshawe El Oakley, R; Lim, SK
Testing null
Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system.
Tsukiyama T, Asano R, Kawaguchi T, Kim N, Yamada M, Minami N, Ohinata Y, Imai H.
Genes Cells null
A distinct expression pattern in mammalian testes indicates a conserved role for NANOG in spermatogenesis.
Kuijk, EW; de Gier, J; Lopes, SM; Chambers, I; van Pelt, AM; Colenbrander, B; Roelen, BA
Testing null
Kunie Hagiwara et al.
PloS one, 9(1), e84072-e84072 (2014-01-28)
The outstanding differentiation capacities and easier access from adult tissues, cells derived from neural crest cells (NCCs) have fascinated scientists in developmental biology and regenerative medicine. Differentiation potentials of NCCs are known to depend on their originating regions. Here, we
Gaelen S Smith et al.
Biomolecules, 3(3), 351-368 (2014-03-13)
Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid that activates a family of G protein coupled-receptors (GPCRs) implicated in mammalian development, angiogenesis, immunity and tissue regeneration. S1P functions as a trophic factor for many cell types, including embryonic stem cells (ESCs). Sphingosine
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As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

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SKUGTIN
AB573104053252286810
AB5731-25UG04054839342684

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