Anti-FTO Antibody, clone 5-2H10

Alpha-ketoglutarate-dependent dioxygenase FTO (Fat mass and obesity-associated protein) is a ubiquitous nuclear protein which belongs to the FTO family. It is a dioxygenase enzyme that dealkylates nucleic acids by oxidative demethylation in the presence of oxygen, alpha-ketoglutarate and iron (Fe2+). FTO is highly active on single-stranded RNA molecules with 3-methyluracil residues and on single-stranded DNA containing 3-methylthymine residues, and has relatively low activity on single-stranded DNA containing either 3-methylcytosine or 3-methyladenine. There has been no observed activity on double-stranded DNA. FTO is highly abundant in the hypothalamus, pituitary, and adrenal glands. It plays an important role in energy homeostasis and the regulation of fat accumulation in the body, and may be involved in obesity. Mutated FTO proteins are linked to a syndrome characterized by retarded growth, delayed development, coarse facies and early death.

~58 kDa observed.

Recombinant protein corresponding to human FTO.

Anti-FTO Antibody, clone 5-2H10 detects level of FTO & has been published & validated for use in Western Blotting, IHC(P).

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected FTO in glandular cells of rat pituitary gland tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Human kidney tissue lysate

Evaluated by Western Blot in human kidney tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected FTO in 10 µg of human kidney tissue lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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