Anti-Ago2 Antibody, clone 11A9

Ago2 (argonaute-2), also known as eukaryotic translation initiation factor 2C (EIF2C2), is an essential component for siRNA-directed RNA interference (RNAi) response. Ago2 is an endonuclease required for the unwinding of siRNA duplex and assembly of siRNA into RNA-induced silencing complexes (RISC). Ago2 interacts with DICER1 through its Piwi domain. This Piwi domain is thought to provide RNA cleavage activity via a mechanism similar to RNase H. Ago2 activity is necessary for embryonic development as well as RNA-mediated gene silencing (RNAi).

~85 kDa observed. Two isoforms at ~97 kDa and ~94 kDa are known to exist.

Linear peptide corresponding to human Ago2.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Ago2 in HEK239 cell lysate (Rudel, S., et al. (2008) RNA. 14(6):1244-1253.).

Immunocytochemistry Analysis: A represeentative lot from an independent laboratory detected Ago2 in HEK293, HeLa, and RPE-1 cells (Weinmann, L., et al. (2009). Cell. 136(3):496-507.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Use Anti-Ago2 Antibody, clone 11A9 (Rat Monoclonal Antibody) validated in WB, IP, ICC to detect Ago2 also known as Protein argonaute-2, Argonaute2, hAgo2, eIF-2C 2.

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected Ago2 in 10 µg of HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.