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UNSPSC Code:
41106305
NACRES:
NA.54
Form:
lyophilized
Solubility:
water: soluble
Storage temp.:
2-8°C
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Polyadenylic acid
form
lyophilized
mol wt
700-3500 kDa
packaging
pkg of 100 mg
manufacturer/tradename
Roche
concentration
0.5 mg/mL (Working concentration)
technique(s)
PCR: suitable
color
white
solubility
water: soluble
absorbance ratio
A290/260 nm 0.03-0.05, A280/260 nm 0.28-0.32, A250/260 nm 0.86-0.90
storage temp.
2-8°C
Quality Level
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General description
Poly(A) is used as a carrier for quantitative precipitation of DNA and RNA.
Application
Poly(A) has been used for droplet digital PCR (ddPCR) assay.
Polyadenylic acid (Poly(A)) to inhibit Exo1′s exonuclease activity. It has also been used as a carrier to resuspend lyophilized gBlocks.
Biochem/physiol Actions
Polyadenylic acid (Poly(A)) tails present at 3′ end are produced in the cell nucleus. It contains ~250 nucleotides in mammalian cells. Poly(A) regulates mRNA decay and the initiation of translation. Cytoplasmic poly(A) extension modulates translation.
Physical form
Lyophilizate, potassium salt
Preparation Note
Working concentration: 0.5 mg/ml
A concentration of 0.5 mg/ml is recommended.
Working solution: 0.5 mg/ml is recommended.
A concentration of 0.5 mg/ml is recommended.
Working solution: 0.5 mg/ml is recommended.
Analysis Note
Typical analysis:
2.3μmol/mg Poly(A) (from absorbance) in relation to one mononucleotide unit. Chromatographically homogeneous.
Absorbance determination A250/A260, A280/A260, A290/A260
2.3μmol/mg Poly(A) (from absorbance) in relation to one mononucleotide unit. Chromatographically homogeneous.
Absorbance determination A250/A260, A280/A260, A290/A260
Other Notes
1 A260 unit corresponds to 40 μg ssRNA.
Chain Length 2.100 to 10.000 nucleotides
For life science research only. Not for use in diagnostic procedures.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Poly (A) tail length is controlled by the nuclear poly (A)-binding protein regulating the interaction between poly (A) polymerase and the cleavage and polyadenylation specificity factor
Kuhn U, et al.
The Journal of Biological Chemistry, 284(34), 22803-22814 (2009)
Yuichiro Miyaoka et al.
Scientific reports, 6, 23549-23549 (2016-04-01)
Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA nicking or double-strand breaks (DSBs) by homology-directed repair (HDR). However, nonhomologous end-joining (NHEJ), an error-prone repair, acts concurrently, reducing the rate of high-fidelity edits. The identification of genome-editing conditions that
Poly (ADP-ribose)-binding promotes Exo1 damage recruitment and suppresses its nuclease activities
Cheruiyot A, et al.
DNA Repair, 35, 106-115 (2015)
Kengo Watanabe et al.
Nature communications, 12(1), 1353-1353 (2021-03-03)
Cells are under threat of osmotic perturbation; cell volume maintenance is critical in cerebral edema, inflammation and aging, in which prominent changes in intracellular or extracellular osmolality emerge. After osmotic stress-enforced cell swelling or shrinkage, the cells regulate intracellular osmolality
Peiguo Yang et al.
Cell, 181(2), 325-345 (2020-04-18)
The mechanisms underlying ribonucleoprotein (RNP) granule assembly, including the basis for establishing and maintaining RNP granules with distinct composition, are unknown. One prominent type of RNP granule is the stress granule (SG), a dynamic and reversible cytoplasmic assembly formed in
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