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NAD100-RO

Roche

βDinucleótido de β-nicotinamida y adenina hydrate

Grade I, free acid

Sinónimos:

β-DPN, β-NAD, -NAD, Coenzima 1, Cozimasa, DPN, Nadide, Nucleótido de difosfopiridina

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Fórmula empírica (notación de Hill):
C21H27N7O14P2 · xH2O
Número CAS:
53-84-9
Peso molecular:
663.43 (anhydrous basis)
UNSPSC Code:
41106305
PubChem Substance ID:
329818699
MDL number:
MFCD00036253
NACRES:
NA.54
eCl@ss:
39200202
Assay:
100% (dry weight)
Form:
lyophilized
Storage temp.:
2-8°C
Servicio técnico
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assay

100% (dry weight)

form

lyophilized

mol wt

Mr 663.4

packaging

pkg of 1 g (10127965001), pkg of 5 g (10127973001)

manufacturer/tradename

Roche

color

off-white

pH

~3.0 (50 mg/mL in water)

λmax

260 nm

shipped in

wet ice

storage temp.

2-8°C

SMILES string

NC1=NC=NC2=C1N=CN2.O[C@@H]3[C@@H](COP(O)(OP(OC[C@@H](O4)[C@@H](O)[C@@H](O)[C@H]4[N+]5=CC=CC(C(N)=O)=C5)([O-])=O)=O)OC[C@@H]3O

InChI

1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1

InChI key

BAWFJGJZGIEFAR-NNYOXOHSSA-N

General description

Nicotinamide adenine dinucleotide (NAD) is a ubiquitously found electron carrier and a cofactor. NAD+ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD+ complexes, the enzyme-cofactor interactions are highly conserved.

Application

NAD (nicotinamide adenine dinucleotide), Grade I has been used in the kinetic studies of human class IV alcohol dehydrogenase.
NAD has been used in in vitro glycogen synthase kinase 3 (GSK3) deacetylation assay. It has also been used as a component in assay mix for the determination of kinetic characteristics using an enzyme-linked kinetic assay.

Biochem/physiol Actions

Aceptor de electrones
The NAD (nicotinamide adenine dinucleotide)/NADH ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo. It works as an electron acceptor.
NAD is also involved in microbial catabolism. β-NAD acts as a substrate for various enzymes in several cellular processes.

Features and Benefits

Absorbance coefficients: 17.6 x 103l x mol-1 x cm-1 at 260nm, 5.9 x 103l x mol-1 x cm-1 at 333nm (CN complex)

Packaging

Envasado por peso sólido.

Preparation Note

Storage conditions (working solution): 10 mg/ml in aqueous solution, contains at least 99% NAD after 4 weeks at 2 to 8 °C

Analysis Note

Purity: 100% β-NAD based on dry weight (enzymatically, CN-complex and absorbance); A250nm - A260nm : 0.83 ± 0.02, A280nm - A260nm : 0.22 ± 0.02
Absence of contaminants: LDH inhibitors are not detectable
Organic solvents: =0.05% (GC)

Other Notes

Esta es la forma común del NAD.
For life science research only. Not for use in diagnostic procedures.

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Clase de almacenamiento

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 2

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Certificados de análisis (COA)

Lot/Batch Number
86813129
86813124
86813121
82320028
82320027

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N J Hughes et al.
Journal of bacteriology, 180(5), 1119-1128 (1998-03-12)
Helicobacter pylori, a major cause of human gastric disease, is a microaerophilic bacterium that contains neither pyruvate nor 2-oxoglutarate dehydrogenase activity. Previous studies (N. J. Hughes, P. A. Chalk, C. L. Clayton, and D. J. Kelly, J. Bacteriol. 177:3953-3959, 1995)
The kinetic characteristics of human and trypanosomatid phosphofructokinases for the reverse reaction
Fernandes PM, et al.
The Biochemical Journal, 476(2), 179-191 (2019)
Chu-Fang Chou et al.
The Journal of biological chemistry, 277(28), 25209-25216 (2002-05-09)
Molecular genetic studies have indicated that alcohol dehydrogenase may be involved in the synthesis of retinoic acid, a hormonal molecule regulating diverse cellular functions at the transcriptional level. Class IV alcohol dehydrogenase (ADH) has been reported to be the most
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