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Permítanos ayudarleNombre del producto
Amyloglucosidase, from Aspergillus niger
biological source
Aspergillus niger
form
lyophilized
specific activity
6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate)
~6 units/mg protein (At 25 °C with glycogen as the substrate.)
mol wt
97 kDa
packaging
pkg of 3,500 U (11202367001)
pkg of 500 U (11202332001)
manufacturer/tradename
Roche
optimum pH
4.6-4.8
storage temp.
2-8°C
Quality Level
Categorías relacionadas
Application
Amyloglucosidase from Aspergillus niger is used for the determination of starch.
Biochem/physiol Actions
Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4) and α-(1,6) glucosidic bonds of starch and malto-oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.
Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.
General description
Amyloglucosidase, a 1,4-α-D-glucan glucohydrolase, is a disaccharidase–type α-glucosidase. It is synthesized by several Aspergillus genus species. This exo-enzyme is one of the major industrial enzymes. The stability of amyloglucosidase can be increased by immobilization.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Preparation Note
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
Working solution: Tris-buffered saline containing 0.1% Tween 20.
Storage conditions (working solution): 2 to 8 °C
The following concentrations should be taken as a guideline:
- Western blot: 1 to 10 μg/ml
Working solution: Tris-buffered saline containing 0.1% Tween 20.
Storage conditions (working solution): 2 to 8 °C
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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High-potency amyloglucosidase-producing mold of the Aspergillus niger group.
Smiley K L, et al.
Applied Microbiology, 12(5), 455-455 (1964)
Simultaneous amyloglucosidase and exo-polygalacturonase production by Aspergillus niger using solid-state fermentation.
Costa, J et al.
Brazilian Archives of Biology and Technology, 50, 759-766 (2007)
High fructose corn syrup: production, uses and public health concerns.
Parker K, et al.
Biotechnology and Molecular Biology Reviews, 5, 71-78 (2010)
Boaz Hilman et al.
Plant, cell & environment, 44(8), 2522-2535 (2021-06-08)
Little is known about the sources and age of C respired by tree roots. Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool
The action of an amyloglucosidase of Aspergillus niger on starch and malto-oligosaccharides.
J H PAZUR et al.
The Journal of biological chemistry, 234(8), 1966-1970 (1959-08-01)
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