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Merck

03777

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

BioUltra, ≥99.0% (T)

Sinónimos:

EGTA, Egtazic acid, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid, Glycol ether diamine tetraacetic acid

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About This Item

Fórmula lineal:
[-CH2OCH2CH2N(CH2CO2H)2]2
Número CAS:
Peso molecular:
380.35
UNSPSC Code:
12352106
NACRES:
NA.25
PubChem Substance ID:
EC Number:
200-651-2
Beilstein/REAXYS Number:
1717370
MDL number:

Nombre del producto

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid, BioUltra, ≥99.0% (T)

InChI key

DEFVIWRASFVYLL-UHFFFAOYSA-N

InChI

1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)

SMILES string

OC(=O)CN(CCOCCOCCN(CC(O)=O)CC(O)=O)CC(O)=O

grade

Molecular Biology

product line

BioUltra

assay

≥99.0% (T)

form

powder

reaction suitability

reagent type: chelator

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

ign. residue

≤0.05% (as SO4)

loss

≤1.0% loss on drying, 110 °C

mp

241 °C (dec.) (lit.)

solubility

0.1 M NaOH: 0.1 M at 20 °C, clear, colorless

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤200 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

absorption

cut-off at 263 nm in 1 M NaOH at 0.1 M

Quality Level

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Application

A chelating agent useful for the determination of calcium in the presence of magnesium.
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid can be used to study molecular biology, biochemicals, reagents and chelators. Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid has been used in a study that illustrated the response of the β-cyclodextrin-hemin complex in the induction of cucumber adventitious rooting through heme oxygenase-1-dependent mechanism and calcium signaling.

Biochem/physiol Actions

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid A is a chelating agent used for the determination of calcium in the presence of magnesium.

Other Notes

Buffer component in dideoxy sequencing of RNA using reverse transcriptase.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Visite la Librería de documentos

C.S. Hahn et al.
Methods in Enzymology, 180, 123-123 (1989)
Kennedy R Lees et al.
Stroke, 44(3), 580-584 (2013-02-09)
DP-b99, a lipophilic moderate-affinity chelator of zinc, was postulated to improve recovery after acute ischemic stroke. We evaluated the safety and therapeutic effects of DP-b99 in patients with acute hemispheric ischemic stroke. The Membrane-Activated Chelator Stroke Intervention trial was a
Walter Marcotti et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(16), 5505-5514 (2014-04-18)
Tip links between adjacent stereocilia are believed to gate mechano-electrical transducer (MET) channels and mediate the electrical responses of sensory hair cells. We found that mouse auditory hair cells that lack tip links due to genetic mutations or exposure to
David R Whitfield et al.
The American journal of geriatric psychiatry : official journal of the American Association for Geriatric Psychiatry, 23(2), 141-148 (2014-06-24)
Depression is a common symptom in dementia with Lewy bodies (DLB), Parkinson disease dementia (PDD), and Alzheimer disease (AD), yet its molecular basis remains unclear and current antidepressants do not appear to be effective. Cerebral zinc has been implicated in
Anita C Bellail et al.
Nature communications, 5, 4234-4234 (2014-06-24)
Ubiquitination governs oscillation of cyclin-dependent kinase (CDK) activity through a periodic degradation of cyclins for orderly cell cycle progression; however, the mechanism that maintains the constant CDK protein levels throughout the cell cycle remains unclear. Here we show that CDK6

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