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Merck

93321

TRIS Glycine buffer solution

BioUltra, 10× concentrate

Sinónimos:

Blotting buffer solution

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NACRES:
NA.25
UNSPSC Code:
41105319
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product line

BioUltra

Quality Level

form

liquid

quality

10× concentrate

composition

glycine, 1.92 M , TRIS, 0.25 M

impurities

insoluble matter, passes filter test, proteases, none detected

ign. residue

≤0.2%

pH

8.5-8.7

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, NH4+: ≤200 mg/kg, Na: ≤500 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.1, λ: 280 nm Amax: 0.1

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. TRIS Glycine buffer solution, 10X concentrate is diluted to a working concentration of 1X and pH adjusted as required. TRIS Glycine buffer (TGB) is frequently used in gel electrophoresis and ion-exchange chromatography applications.

Other Notes

Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications


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Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Guo-Min Tan et al.
Journal of chromatography. A, 1098(1-2), 131-137 (2005-11-30)
Ion-exchange electrochromatography with an oscillatory electric field perpendicular to mobile-phase flow driven by pressure (pIEEC) was developed with a column design of rectangle cross-section. The effect of electric field strength on the dynamic binding capacity (DBC) was examined by frontal
E R Frears et al.
Glycoconjugate journal, 16(6), 283-290 (1999-12-01)
IgG carries bi-antennary N-linked glycans which differ in degrees of galactosylation, core fucosylation and bisecting N-acetyl glucosamine. The majority of these are non-sialyated closely related neutral structures which can be resolved by HPLC analysis, but which are difficult to separate
Zhixin Wang et al.
Electrophoresis, 29(22), 4454-4462 (2008-11-28)
Multiple labeling of nucleic acids by intercalative dyes is a promising method for ultrasensitive nucleic acid assays. The properties of the fast dissociation and instability of dye-DNA complexes may prevent from their wide applications in CE-LIF nucleic acid analysis. Here