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Merck

95290

Methyl Blue

suitable for microscopy

Sinónimos:

Acid blue 93, Aniline blue water soluble, Cotton blue, Poirriers blue, Water blue

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Fórmula empírica (notación de Hill):
C37H27N3Na2O9S3
Número CAS:
28983-56-4
Peso molecular:
799.80
UNSPSC Code:
12171500
NACRES:
MA.02
PubChem Substance ID:
329770433
EC Number:
249-352-9
Colour Index Number:
42780
MDL number:
MFCD00058509

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Nombre del producto

Methyl Blue, suitable for microscopy

InChI

1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2

InChI key

MCPLVIGCWWTHFH-UHFFFAOYSA-L

SMILES string

[Na+].[Na+].OS(=O)(=O)c1ccc(Nc2ccc(cc2)\C(c3ccc(Nc4ccc(cc4)S([O-])(=O)=O)cc3)=C5/C=CC(\C=C5)=N/c6ccc(cc6)S([O-])(=O)=O)cc1

form

powder or crystals

technique(s)

microbe id | staining: suitable

pH

9.4-14

solubility

water: 0.01 g/10 mL, blue to very deep blue

εmax

≥200 at 310-320 nm
≥400 at 595-605 nm

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Methyl Blue has been used:
  • to stain leaf disks for callose visualization within the cells
  • to incubate cells for 4′,6-diamidino-2-phenylindole (DAPI)/septa staining
  • as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin
  • in Mann′s stain as it involves a methyl blue-eosin mixture

General description

Methyl Blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.

Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

>572.0 °F - Pensky-Martens closed cup

flash_point_c

> 300 °C - Pensky-Martens closed cup

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
BCCK8203
BCCB9353
BCBV2236
BCBR0621V

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Sebastian Thaler et al.
Retina (Philadelphia, Pa.), 29(9), 1257-1265 (2009-11-26)
To investigate the biocompatibility of methyl blue and aniline blue as vital dyes for vitreoretinal surgery in an in vivo rat model and to evaluate the effect of these dyes on retinal structure and function. Adult Brown-Norway rats received intravitreal
M Safaríková et al.
Chemosphere, 59(6), 831-835 (2005-04-07)
Brewer's yeast (bottom yeast, Saccharomyces cerevisiae subsp. uvarum) cells were magnetically modified using water based magnetic fluid stabilized with perchloric acid. Magnetically modified yeast cells efficiently adsorbed various water soluble dyes. The dyes adsorption can be described by the Langmuir
Yu-Lin Kuo et al.
Journal of hazardous materials, 190(1-3), 938-944 (2011-05-11)
An optimal condition applied to the Taguchi method with an L(9) orthogonal array for preparing a visible-light driven nitrogen-modified TiO(2) (N-TiO(2)) photocatalyst by a simple hydrolysis method has been examined for material characteristics and a photodecolorization test of methyl blue
Lulu Fan et al.
International journal of biological macromolecules, 50(2), 444-450 (2012-01-10)
A novel nano-adsorbent, β-cyclodextrin-chitosan (CDC) modified Fe(3)O(4) nanoparticles (CDCM) is fabricated for removal of methyl blue (MB) from aqueous solution by grafting CDC onto the magnetite surface. The characteristics results of FTIR, SEM and XRD show that CDC is grafted
Peng Song et al.
Nanoscale, 4(5), 1800-1804 (2012-02-03)
Novel nanocomposite films, based on graphene oxide (GO) and TiO(2) nanotube arrays, were synthesized by assembling GO on the surface of self-organized TiO(2) nanotube arrays through a simple impregnation method. The composite films were characterized with field emission scanning electron
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