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Merck

97622

2- Mercaptoetanol

derivatization grade (HPLC), LiChropur™, ≥99.0% (GC)

Sinónimos:

β-Mercaptoetanol, 2- Hidroxietilmercaptano, BME, Tioetilenglicol

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About This Item

Fórmula lineal:
HSCH2CH2OH
Número CAS:
60-24-2
Peso molecular:
78.13
UNSPSC Code:
23151817
NACRES:
NA.05
PubChem Substance ID:
329770550
EC Number:
200-464-6
Beilstein/REAXYS Number:
773648
MDL number:
MFCD00004890

Nombre del producto

2- Mercaptoetanol, derivatization grade (HPLC), LiChropur™, ≥99.0% (GC)

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

SMILES string

OCCS

grade

derivatization grade (HPLC)

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

assay

≥99.0% (GC)

form

liquid

quality

LiChropur™

expl. lim.

18 %

concentration

14.3 M (pure liquid)

technique(s)

HPLC: suitable

refractive index

n20/D 1.500 (lit.)
n20/D 1.500-1.502

bp

157 °C (lit.)

density

1.114 g/mL at 25 °C (lit.)

storage temp.

2-8°C

Quality Level

100

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Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
suitable for derivatisation of Amine groups

General description

2-Mercaptoethanol is a thiol compound showing growth promoting properties on mouse lymphoma L1210 cells in vitro. It also enhances viability, blast transformation and antibody formation in lymphocyte cultures.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Liver,Heart

Clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

165.2 °F - closed cup

flash_point_c

74 °C - closed cup

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Certificados de análisis (COA)

Lot/Batch Number
BCCN7818
BCCN2095
BCCM7502
BCCL6859
BCCK0471

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Mechanism of growth stimulation of L1210 cells by 2-mercaptoethanol in vitro. Role of the mixed disulfide of 2-mercaptoethanol and cysteine
Ishii T, et al.
The Journal of Biological Chemistry, 256(23), 12387-12392 (1981)
Katharina Rothe et al.
Blood, 123(23), 3622-3634 (2014-04-24)
Previous studies demonstrated that imatinib mesylate (IM) induces autophagy in chronic myeloid leukemia (CML) and that this process is critical to cell survival upon therapy. However, it is not known if the autophagic process differs at basal levels between CML
Leon Tribolet et al.
The Journal of infectious diseases, 211(3), 416-425 (2014-08-21)
Na-ASP-2 is an efficacious hookworm vaccine antigen. However, despite elucidation of its crystal structure and studies addressing its immunobiology, the function of Na-ASP-2 has remained elusive. We probed a 9000-protein human proteome microarray with Na-ASP-2 and showed binding to CD79A
Emily Cunningham Williams et al.
Human molecular genetics, 23(11), 2968-2980 (2014-01-15)
The disease mechanism of Rett syndrome (RTT) is not well understood. Studies in RTT mouse models have suggested a non-cell-autonomous role for astrocytes in RTT pathogenesis. However, it is not clear whether this is also true for human RTT astrocytes.
Bethany L Johnson-Kerner et al.
Human molecular genetics, 24(5), 1420-1431 (2014-11-16)
Giant axonal neuropathy (GAN) is a progressive neurodegenerative disease caused by autosomal recessive mutations in the GAN gene resulting in a loss of a ubiquitously expressed protein, gigaxonin. Gene replacement therapy is a promising strategy for treatment of the disease;
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