A5533
Alizarin Red S
certified by the Biological Stain Commission, powder
Sinónimos:
3,4-Dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt, Alizarin Carmine, Alizarin Red, water soluble, Alizarin sodium monosulfonate, Alizarin sulfonate sodium, Alizarinsulfonic acid sodium salt, Sodium alizarinsulfonate
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About This Item
Nombre del producto
Alizarin Red S, certified by the BSC
SMILES string
[Na+].Oc1c(O)c(cc2C(=O)c3ccccc3C(=O)c12)S([O-])(=O)=O
InChI
1S/C14H8O7S.Na/c15-11-6-3-1-2-4-7(6)12(16)10-8(11)5-9(22(19,20)21)13(17)14(10)18;/h1-5,17-18H,(H,19,20,21);/q;+1/p-1
InChI key
HFVAFDPGUJEFBQ-UHFFFAOYSA-M
agency
certified by the BSC
form
powder
concentration
0.015 g/L in 0.1 M NaOH
technique(s)
titration: suitable
color
dark red
yellow-orange to dark orange
solubility
H2O: 1 mg/mL
ε (extinction coefficient)
592-598 nm at 0.015 g/L in 0.1 M NaOH
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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Categorías relacionadas
Application
- Alizarin Red S has been used for checking the calcium deposits in osteogenic culture.
- It has been used for the quantitative analyses of calcification in aortic explants.
- It has been used for mineral staining in osteoblast cultures.
Biochem/physiol Actions
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Product A5533, Alizarin Red S, is commonly used to stain calcium in tissue sections. Will other cations such as magnesium, manganese, barium and strontium also be detected?
No, typically only calcium is present in sufficient quantities to be demonstrated in tissue sections.
Product A5533, Alizarin Red S, has been prepared according to protocol. The solution has gelled or thickened. Is the solution still usable?
Yes. Although the solution has thickened or gelled, it is still suitable for use.
I see that Product A5533, Alizarin Red S, was tested according to the Dahl method at pH 7.0. Can the dye also be used in the McGee-Russell procedure at pH 4.2?
Yes. The dye can be used at a variety of pH values.
What are the pH transition ranges for Product A5533, Alizarin Red S?
Although the pH transition ranges are not determined on a lot to lot basis, the solution changes from yellow to red in the range of 3.5-6.5, and from the orange to violet at pH 9.4-12. On a lot to lot basis there can be considerable difference in regards to color and pH ranges.
When using Product A5533, Alizarin Red S, will amorphous calcium deposits be detected in tissue sections?
Amorphous calcium phosphate deposits are very soluble below pH 8.5. In most instances the deposits are lost during either standard fixation or the during the standard staining procedure.
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