E7889
Ethylenediaminetetraacetic acid disodium salt solution
Molecular Biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected
Sinónimos:
(Ethylenedinitrilo)tetraacetic acid disodium salt, EDTA disodium salt, EDTA-Na2
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Fórmula lineal:
[-CH2N(CH2CO2Na)CH2CO2H]2
Número CAS:
Peso molecular:
336.21
UNSPSC Code:
12352107
NACRES:
NA.31
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3822669
Servicio técnico
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Molecular Biology
Quality Level
sterility
0.2 μm filtered
form
liquid
reaction suitability
reagent type: chelator
concentration
0.5 M in H2O
pH
7.5-8.5
suitability
suitable for molecular biology
application(s)
microbiology
foreign activity
DNase, RNase, NICKase and protease, none detected
SMILES string
[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
InChI
1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2
InChI key
ZGTMUACCHSMWAC-UHFFFAOYSA-L
General description
Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid. The four carboxylic acid and two amine groups with lone-pair electrons of EDTA can chelate calcium and several other metal ions.
Ethylenediaminetetraacetic acid disodium salt solution is a chelating agent suitable for molecular biology applications.
Application
Ethylenediaminetetraacetic acid disodium salt solution has been used:
- in Tris hydrochloric acid (HCl) for digesting proteinase-K to determine the amount of DNA in the corneal sample
- with papain to digest corneal samples for the quantification of glycosaminoglycan (GAG) content
- in pretreatment for detaching epithelial cells grown to 80% confluency
- to prepare stock solutions for lysis buffer
- in the preparation of urea lysis buffer
Features and Benefits
molecular biology grade
Preparation Note
Prepared in 18 megohm water with Molecular Biology Reagent EDTA (E 5134)
Other Notes
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signalword
Warning
hcodes
pcodes
Hazard Classifications
STOT RE 2 Inhalation
target_organs
Respiratory Tract
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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