48275
β-Galactosidasa from Escherichia coli
suitable for enzyme immunoassay, lyophilized, powder, ~140 U/mg
Sinónimos:
β-D-galactósido galactohidrolasa, Lactasa
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About This Item
Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
Nombre del producto
β-Galactosidasa from Escherichia coli, suitable for enzyme immunoassay, lyophilized, powder, ~140 U/mg
form
powder
quality
lyophilized
specific activity
~140 U/mg
mol wt
Mr ~135000
contains
phosphate buffer as stabilizer
sucrose as stabilizer
technique(s)
enzyme immunoassay: suitable
shipped in
wet ice
storage temp.
−20°C
Quality Level
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Categorías relacionadas
Biochem/physiol Actions
βLa β-galactosidasa hidroliza la lactosa en sus componentes monosacáridos, glucosa y galactosa. También cataliza la transglucosilación de la glucosa en alolactosa, el inductor de la β-galactosidasa, en un bucle de retroalimentación.
General description
Peso molecular del tetrámero 465 kDa (subunidades de 116,3 kDa cada una)
Application
Aqueous solution may be used directly for derivatization without previous dialysis or gel permeation chromatography.
Other Notes
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 2-nitrophenyl-β-D-galactopyranoside (Cat. No. 73660) per minute at pH 7.8 and 37 °C
Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Catalog No. 69590) ; Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay technique ; Enzyme immunoassay of thyrptropin releasing hormone (TRH) ; Preparation of enzyme-antibody conjugates.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Comparison of two methods of preparing enzyme-antibody conjugates: application of these conjugates for enzyme immunoassay.
M J O'Sullivan et al.
Analytical biochemistry, 100(1), 100-108 (1979-11-15)
M Imagawa et al.
Annals of clinical biochemistry, 21 ( Pt 4), 310-317 (1984-07-01)
beta-D-galactosidase from Escherichia coli and horseradish peroxidase were evaluated as labels of Fab' in dose-response curves for human alpha-fetoprotein and human chorionic gonadotropin by sandwich enzyme immunoassay technique using fluorogenic substrates for enzyme assay. The non-specific binding of Fab'-peroxidase conjugates
K Kato et al.
European journal of biochemistry, 62(2), 285-292 (1976-02-16)
Rabbit immunoglobulin G (IgG) was reduced by incubating with 10 mM 2-mercaptoethylamine and then treated with excess amounts of N,N'-o-phenylenedimaleimide. As a result, maleimide residues were introduced into rabbit IgG molecules. Rabbit IgG containing maleimide residues could be coupled to
M Takeyama et al.
Chemical & pharmaceutical bulletin, 40(8), 2199-2201 (1992-08-01)
A sensitive and specific double-antibody enzyme immunoassay (EIA) for a thyrotropin releasing hormone (TRH)-like immunoreactive substance has been developed. In order to synthesize TRH-labeled beta-D-galactosidase (beta-gal), a newly devised TRH derivative, pGlu-His-Pro-NH-(CH2)6-NH2 (TRH-Hex), was employed. TRH-Hex was linked to beta-gal
J W Callahan
Biochimica et biophysica acta, 1455(2-3), 85-103 (1999-11-26)
GM1 gangliosidosis and Morquio B disease are distinct disorders both clinically and biochemically yet they arise from the same beta-galactosidase enzyme deficiency. On the other hand, galactosialidosis and sialidosis share common clinical and biochemical features, yet they arise from two
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