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Merck

71183

Sodium acetate

anhydrous, BioUltra, suitable for luminescence, Molecular Biology, ≥99.0% (NT)

Sinónimos:

Sodium acetate anhydrous, Acetic acid Sodium salt anhydrous, Acetic acid sodium salt

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About This Item

Fórmula lineal:
CH3COONa
Número CAS:
Peso molecular:
82.03
NACRES:
NA.25
PubChem Substance ID:
eCl@ss:
39021906
UNSPSC Code:
12161700
EC Number:
204-823-8
MDL number:
Beilstein/REAXYS Number:
3595639

Nombre del producto

Sodium acetate, anhydrous, BioUltra, suitable for luminescence, Molecular Biology, ≥99.0% (NT)

InChI key

VMHLLURERBWHNL-UHFFFAOYSA-M

InChI

1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1

SMILES string

[Na+].CC([O-])=O

grade

Molecular Biology

product line

BioUltra

assay

≥99.0% (NT)

form

solid

autoignition temp.

1112 °F

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

loss

≤0.5% loss on drying, 150 °C

pH

8.5-10.0 (25 °C, 3 M in H2O)

pKa 

4.76 (acetic acid)

mp

>300 °C (dec.) (lit.)

solubility

H2O: 3 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤20 mg/kg
phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤20 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤10 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤200 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤10 mg/kg
Zn: ≤5 mg/kg

λ

3 M in H2O

UV absorption

λ: 260 nm Amax: 0.03
λ: 280 nm Amax: 0.02

suitability

suitable for luminescence

Quality Level

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Application

Sodium acetate is suitable for use as a buffer in:
  • protein crystallization
  • staining of gels in protein gel, electrophoresis, neutralization buffer for chromatography
  • the preparation of standard (acetylcholine chloride) for hestrin assay
  • substrate screening characterization for microfluidic microbial fuel cell array

Biochem/physiol Actions

Sodium acetate anhydrous is commonly used in molecular biology applications. This serves as a buffering agent in the DNA digestion experiments by ensuring the reaction has optimum pH. In general sodium acetate is preferred in purification, precipitation, crystallization, and other applications which require tight control of elemental content. Sodium acetate is extensively used as a retardant in plastics manufacturing, as a mordant in dyeing, and in the tanning of leather.

Other Notes

Extraction buffer component in the large-scale phenol extraction of RNA (for mRNA isolation).

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Microfluidic microbial fuel cell array for multiplexed long-term parallel analysis of microbial activities
Hou H, et al.
15th international conference on miniaturized systems for chemistry and life sciences, Seattle, Washington (2011)
O-acetylation of typhoid capsular polysaccharide confers polysaccharide rigidity and immunodominance by masking additional epitopes
Hitri K, et al.
Vaccine (2019)
Enzyme Crystals and Hydrogel Composite Membranes as New Active Food Packaging Material
Mirabelli V, et al.
Global challenges (Hoboken, NJ) (2018)
Determination of the G+ C content of prokaryotes
Mesbah NM, et al.
Methods in Microbiology (2011)
Richard A Willis et al.
Current protocols, 1(2), e36-e36 (2021-02-05)
Class II major histocompatibility complex peptide (MHC-IIp) multimers are precisely engineered reagents used to detect T cells specific for antigens from pathogens, tumors, and self-proteins. While the related Class I MHC/peptide (MHC-Ip) multimers are usually produced from subunits expressed in

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