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Triton™ X-114

Sinónimos:

(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, Polyethylene glycol tert-octylphenyl ether

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Fórmula lineal:
(C2H4O)n C14H22O, n = 7 or 8
Número CAS:
9036-19-5
UNSPSC Code:
12161900
NACRES:
NA.21
PubChem Substance ID:
57653556
MDL number:
MFCD00128254
Beilstein/REAXYS Number:
2315025
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description

non-ionic

Quality Level

200

form

liquid

mol wt

average mol wt 537

impurities

≤0.001% peroxides (as H2O2), ≤1% water

ign. residue

≤0.2%

refractive index

n20/D 1.488 (lit.), n20/D 1.495

CMC

0.2 mM (20-25°C)

transition temp

pour point ~−9 °C, cloud point 23 °C

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤15 mg/kg, K: ≤500 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤500 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg

HLB

12.4

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

General description

Triton™ X-114 is a nonionic detergent. It is a good detergent for solubilizing membranes having reversible phase-separation property useful for the separation of intrinsic membrane proteins. Solution of Triton™ X-114 is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C. Phase separation of proteins in solutions of Triton™ X-114 has been investigated. This octylphenol ethoxylate is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic & alkaline solutions.

Application

Triton™ X-114 has been used:
  • In phase separation of integral membrane proteins
  • In the purification of bacterial inclusion bodies
  • To study the clouding phenomenon in promethazine hydrochloride, an amphiphilic phenothiazine drug
  • To partition conditioned media into insoluble and soluble phases before Western blotting
  • For solubilization of phosphatidylinositol 4-phosphate 5-kinase from bovine brain

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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Danger

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

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Certificados de análisis (COA)

Lot/Batch Number
MKCZ8321
MKCV9212
MKCT8848
MKCS2583
MKCP2867

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Identification of a new 80 k isoform of phosphatidylinositol 4-phosphate 5-kinase from bovine brain.
Schmidt M, et al.
Febs Letters, 325(3), 201-204 (1993)
J.G. Pryde
Trends in Biochemical Sciences, 11, 160-160 (1986)
A Sánchez-Ferrer et al.
Plant physiology, 91(4), 1481-1487 (1989-12-01)
Polyphenoloxidase from grape berries is extracted only by nonionic detergents with a hydrophilic-lipophilic balance between 12.4 and 13.5. The enzyme was partially purified in latent form, free of phenolics and chlorophylls, by using temperature phase partitioning in a solution of
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