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Merck

A2512

Albúmina from chicken egg white

lyophilized powder, ≥98% (agarose gel electrophoresis)

Sinónimos:

Albúmina from hen egg white, Ovalbúmina

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Número CAS:
UNSPSC Code:
12352202
eCl@ss:
42010201
EC Number:
232-692-7
NACRES:
NA.61
MDL number:
Biological source:
chicken egg white
Assay:
≥98% (agarose gel electrophoresis)
Form:
lyophilized powder
Technique(s):
bioconjugation: suitable
Concentration:
4—20 mol/mol albumin
Servicio técnico
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biological source

chicken egg white

Quality Level

type

Grade VI

assay

≥98% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

44287 Da by calculation

purified by

chromatography

concentration

4—20 mol/mol albumin

extent of labeling

4-20 mol mannose per mol ovalbumin protein

technique(s)

bioconjugation: suitable

solubility

H2O: soluble 50 mg/mL, clear to slightly hazy, colorless to faintly yellow

UniProt accession no.

storage temp.

2-8°C

Gene Information

chicken ... OVAL(396058)

General description

Ovalbumin makes 75% of the total egg white protein.
Chicken egg albumin is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated-glycoprotein. From the amino acid sequence, the peptide portion of the molecule consists of 385 residues and has a molecular weight of 42.7 kDa.

Application

Glucoproteína fosforilada que es útil como marcador (~45 kDa) para la electroforesis de proteínas. Se ha utilizado para producir un modelo animal del asma.
Ovalbumin has been used:
  • as a medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophil
  • to induce allergic inflammation and atopic dermatitis in experimental mice
  • as a component in control mixture for validating tandem mass spectral analysis
  • as a model protein antigen to evaluate the in vivo efficacy of a chitosan/interleukin (IL)-12 adjuvant system

Biochem/physiol Actions

Albumin from chicken egg white serves as a natural adhesive.
Differential scanning calorimetry indicates that chicken egg albumin denatures at 84 °C. Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen. It has the following amino acids: 20 Lys, 10 Tyr, 6 Cys, 14 Asp, and 33 Glu which make it suitable for conjugation.

Physical form

Essentially salt free

Preparation Note

A further purification of A 5503 to reduce mannose content.


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pictograms

Health hazard

signalword

Danger

hcodes

Clase de almacenamiento

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Hazard Classifications

Resp. Sens. 1



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