A3553

Acrilamida

Name: Acrilamida
Brand: SIGMA

suitable for electrophoresis, ≥99% (HPLC), powder

Sinónimos:

2-Propenamida, Amida del ácido acrílico

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About This Item

Fórmula lineal:

CH₂=CHCONH₂

Número CAS:

79-06-1

Peso molecular:

71.08

NACRES:

NB.22

PubChem Substance ID:

24890783

eCl@ss:

39031205

UNSPSC Code:

41105319

EC Number:

201-173-7

MDL number:

MFCD00008032

Beilstein/REAXYS Number:

605349

Nombre del producto

Acrilamida, suitable for electrophoresis, ≥99% (HPLC), powder

cation traces

Fe: ≤1 ppm
Mg: ≤3 ppm
heavy metals (as Pb): ≤5 ppm

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

SMILES string

NC(=O)C=C

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

assay

≥99% (HPLC)

form

powder

technique(s)

electrophoresis: suitable

impurities

≤0.005% Acrylic acid
≤0.005% Insoluble in water

conductivity

≤10 μmho (35% solution)

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

Quality Level

200

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Application

Acrylamide has been used in the preparation of polyacrylamide gels.

General description

Acrylamide functions as a quencher of tryptophan fluorescence. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules. Bis-acrylamide is essential for crosslinking and acrylamide is required for the polymerization of monomers. Acrylamide alkylates SH groups of cysteine and ε-NH₂ lysine residues of proteins during electrophoresis.

Other Notes

This substance has been tested against several types of hand protection for CE compliance. See Related Products to find the recommended gloves for handling this product.

pictograms

GHS06,GHS08

signalword

Danger

hcodes

H301,H312 + H332,H315,H317,H319,H340,H350,H361f,H372

pcodes

P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

280.4 °F - closed cup

flash_point_c

138 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificados de análisis (COA)

Lot/Batch Number

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Visite la Librería de documentos

The Protein Protocols Handbook (2007)

Relationships among cell morphology, intrinsic cell stiffness and cell-substrate interactions

Chiang MYM, et al.

Biomaterials, 34(38), 9754-9762 (2013)

Chemistry, biochemistry, and safety of acrylamide. A review

Friedman M

Journal of Agricultural and Food Chemistry, 51(16), 4504-4526 (2003)

Composite three-dimensional woven scaffolds with interpenetrating network hydrogels to create functional synthetic articular cartilage.

I-Chien Liao et al.

Advanced functional materials, 23(47), 5833-5839 (2014-03-01)

The development of synthetic biomaterials that possess mechanical properties that mimic those of native tissues remains an important challenge to the field of materials. In particular, articular cartilage is a complex nonlinear, viscoelastic, and anisotropic material that exhibits a very

Acrylamide concentration determines the direction and magnitude of helical membrane protein gel shifts.

Arianna Rath et al.

Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15668-15673 (2013-09-11)

SDS/PAGE is universally used in biochemistry, cell biology, and immunology to resolve minute protein amounts readily from tissue and cell extracts. Although molecular weights of water-soluble proteins are reliably determined from their SDS/PAGE mobility, most helical membrane proteins, which comprise

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Product Information Sheet - A3553

Product Information Sheet

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