A6003
Seroalbúmina bovina
lyophilized powder, essentially fatty acid free, ≥96% (agarose gel electrophoresis)
Sinónimos:
Albúmina bovina, Albúmina sérica bovina, BSA
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About This Item
Nombre del producto
Seroalbúmina bovina, lyophilized powder, essentially fatty acid free, ≥96% (agarose gel electrophoresis)
biological source
bovine
assay
≥96% (agarose gel electrophoresis)
≥96%
form
lyophilized powder
mol wt
~66 kDa
purified by
cold ethanol fractionation
packaging
poly bottle of
origin
USA origin
free fatty acid content
≤0.01%
technique(s)
microbiological culture: suitable
impurities
≤0.01% fatty acid (GC)
pH
7
solubility
water: soluble (50 mg/ml)
UniProt accession no.
application(s)
hematology
foreign activity
BT virus, none detected
VSV virus, none detected
storage temp.
2-8°C
Quality Level
Gene Information
bovine ... ALB(280717)
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Categorías relacionadas
Biochem/physiol Actions
Se sospecha que ciertos epítopos conformacionales y de secuencia primaria son alérgenos en las alergias humanas a la leche y a la carne.
Preparation Note
La seroalbúmina puede denominarse también Fracción V. Esta convención de nomenclatura procede del método original de fraccionamiento de las proteínas séricas de Cohn en el que se utiliza precipitación en etanol frío. Utilizando dicho método, la seroalbúmina se encontró en la fracción quinta del etanol. Desde entonces, algunos han venido utilizando la expresión «Fracción V» para describir la albúmina sérica, al margen del método de preparación. Otros han utilizado este término para describir la seroalbúmina purificada por métodos de fraccionamiento en etanol que se han modificado mucho desde que se describió el método Cohn original. Sigma-Aldrich fabrica y distribuye seroalbúminas purificadas mediante una variedad de métodos primarios, entre ellos el verdadero método de fraccionamiento de Cohn, los métodos de fraccionamiento en etanol modificados, el fraccionamiento térmico y la cromatografía. Etapas de purificación añadidas pueden ser la cristalización o la filtración en carbón activado.
Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.
Features and Benefits
- Cold ethanol fractionated
- Essentially fatty acid-free
General description
BSA is a known carrier of fatty acids (FA). Thus control over the specific FA′s for culture of cell lines is important, as different cell lines can be differentially sensitive to particular fatty acids. Fatty acid-free BSA thus is a useful tool for cell culture experiments where the specific fatty acid content must be tightly controlled, to allow researchers to use particular fatty acids specific to their cell lines. Fatty acid-free albumin also allows for optimal and maximum binding sites for adding specific fatty acids to use in cell culture. The use of FA-free BSA also mitigates concerns about endogenous FA′s potentially in non-FA-free BSA.
Bovine serum albumin (BSA) is a highly abundant plasma protein that assists in the regulation of the colloidal osmotic pressure of blood. BSA is used in many different applications, such as cell culture, immunochemistry, and protein quantitation.
This BSA product is specifically tested for very low FA content / fatty acid free status.
This BSA product is specifically tested for very low FA content / fatty acid free status.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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