A6535
Apirasa from potatoes
ATPase ≥200 units/mg protein, lyophilized powder
Sinónimos:
Adenosina 5′-difosfatasa, Adenosina 5′-trifosfatasa
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-569-8
MDL number:
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Permítanos ayudarleNombre del producto
Apirasa from potatoes, ATPase ≥200 units/mg protein, lyophilized powder
type
Grade VII
form
lyophilized powder
ATPase activity
≥200 units/mg protein
secondary activity
≥50 % of base activity ADPase
composition
Protein, 25-60%
solubility
H2O: soluble 1.0 mg/mL
application(s)
diagnostic assay manufacturing
foreign activity
Acid Phosphatase ≤2% of base activity
storage temp.
−20°C
Quality Level
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Application
Se encuentran al menos dos isoenzimas en diferentes variedades de S. tuberosum: una con una elevada relación ATPasa/ADPasa (∼10) y otra con una relación baja (∼1).
Reacción: ATP → ADP+Pi → AMP+2Pi.
Reacción: ATP → ADP+Pi → AMP+2Pi.
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .
Biochem/physiol Actions
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Other Notes
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
This grade has a low ATPase/ADPase ratio.
Packaging
Sold on the basis of ATPase units.
Physical form
Partially purified, lyophilized powder containing potassium succinate buffer salts.
Preparation Note
Derived from red potato
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Traditional in vitro time-kill studies (TKSs) require viable plating, which is tedious and time-consuming. We used ATP bioluminescence, with the removal of extracellular ATP (EC-ATP), as a surrogate for viable plating in TKSs against carbapenem-resistant Gram-negative bacteria (CR-GNB). Twenty-four-hour TKSs
Serena Sirigu et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(47), E7448-E7455 (2016-11-07)
Direct inhibition of smooth muscle myosin (SMM) is a potential means to treat hypercontractile smooth muscle diseases. The selective inhibitor CK-2018571 prevents strong binding to actin and promotes muscle relaxation in vitro and in vivo. The crystal structure of the
Agnete Kirkeby et al.
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The haematopoietic hormone erythropoietin (EPO) has neuroprotective properties and is currently being explored for treatment of stroke and other neurological disorders. Short-term, high-dose treatment with EPO seems to improve neurological function of stroke patients but may be associated with increased
Properties of Two Apyrases from Solanum tuberosum
Kettlun, A. et al.
Phytochemistry, 21(3), 551-558 (1982)
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