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Merck

A8592

ANTI-FLAG® M2-Peroxidasa (HRP) monoclonal antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sinónimos:

ANTI-FLAG® M2 monoclonal antibody produced in mouse, Anti-ddddk, Anti-dykddddk

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About This Item

NACRES:
NA.32
UNSPSC Code:
41106514

Nombre del producto

ANTI-FLAG® M2-Peroxidasa (HRP) monoclonal antibody produced in mouse, clone M2, purified immunoglobulin, buffered aqueous glycerol solution

biological source

mouse

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

species reactivity

all

concentration

~1 mg/mL

technique(s)

indirect ELISA: 1:20,000

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

Gene Information

human ... GPX1(2876)

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Application

Dilución sugerida para inmunocitoquímica e inmunoelectrotransferencia 1:100 a 1:1000
Dilución sugerida para ELISA 1:20 000

Más información sobre los detalles del producto en nuestro portal de aplicación FLAG®.
Para detección sencilla en una etapa mediante inmunocitoquímica, ELISA o inmunoelectrotransferencia (western blot). Especialmente útil en la detección de las proteínas de fusión FLAG expresadas en el huésped murino, donde los anticuerpos anti-ratón secundarios pueden causar reactividad cruzada.

General description

El anticuerpo monoclonal ANTI-FLAG M2-peroxidasa es un anticuerpo IgG covalentemente conjugado a la peroxidasa de rábano picante (HRP). El anticuerpo se une a las proteínas de fusión FLAG y reconoce el epítopo FLAG en los extremos N-terminal, Met-N-terminal, C-terminal y en los péptidos FLAG internos.

Other Notes

Ganador del premio CiteAb 2024 como proveedor de éxito en la investigación del Parkinson

Physical form

Disolución en solución salina tamponada con fosfato que contiene glicerol al 50% más un conservante y un estabilizador.

Preparation Note

Diluir la disolución ANTI- FLAG M2-peroxidasa en disolución salina tamponada con Tris (TBS): Tris 0,05 M, pH 7,4 con NaCl 0,15 M.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1

Clase de almacenamiento

8A - Combustible corrosive hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000505365
0000505363
0000505363
0000505365
0000423147

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  1. Can product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, be used for immunohistochemistry?

    The product cannot be used on paraffin embedded tissue sections, but it does work on fixed culture cells (as indicated on the Product Information Sheet) and also may work on frozen sections.

  2. What concentration of Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, is recommended for my application?

    An antibody titer from 1:100 to 1:1000 is recommended for immunocytochemistry and Western blot.  Minimum antibody titer recommended for ELISA is 1:20,000.  Adjust antibody titer to maximize detection sensitivity and to minimize background.

  3. What are the appropriate storage conditions for Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody?

    This product ships on wet ice and it is recommended to store the undiluted antibody at 0 to -20°C.  Once diluted, repeated freezing and thawing is not recommended.

  4. I am getting a high background on my Western blots using Product No. A8592, Monoclonal Anti-FLAG® M2-Peroxidase (HRP) antibody.  What can I do to fix this?

    There is a troubleshooting guide provided at the end of the product information sheet.  Some suggestions include the following:  adjusting antibody titer, increase number of washes, increase stringency of washes by addition of 0.05% TWEEN 20 or 0.1% TRITON X-100 to the wash buffers, and/or increase the incubation and wash temperatures. 

  5. How is Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, supplied?

    Product No. A8592, ANTI-FLAG M2-peroxidase is supplied as a solution in 10 mM sodium phosphate, pH 7.4, containing 150 mM NaCl and 50% glycerol, plus stabilizer and preservative.  The conjugate protein concentration is approximately 1 mg/ml.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Dong Meng et al.
The Journal of biological chemistry, 284(17), 11425-11435 (2009-01-21)
betaArrestin is a multifunctional signal scaffold protein. Using SPOT immobilized peptide arrays, coupled with scanning alanine substitution and mutagenesis, we show that the MAPK kinase, MEK1, interacts directly with betaarrestin1. Asp(26) and Asp(29) in the N-terminal domain of betaarrestin1 are
Martin Roth et al.
Development (Cambridge, England), 137(9), 1553-1562 (2010-04-02)
FoxG1 is a conserved transcriptional repressor that plays a key role in the specification, proliferation and differentiation of the telencephalon, and is expressed from the earliest stages of telencephalic development through to the adult. How the interaction with co-factors might
Linda Brunotte et al.
Journal of virology, 85(1), 324-333 (2010-10-29)
The 200-kDa L protein of arenaviruses plays a central role in viral genome replication and transcription. This study aimed at providing evidence for the domain structure of L protein by combining bioinformatics with a stepwise mutagenesis approach using the Lassa
Mickaël Lelek et al.
Nature communications, 6, 6483-6483 (2015-03-07)
The molecular mechanisms that allow HIV to integrate into particular sites of the host genome are poorly understood. Here we tested if the nuclear pore complex (NPC) facilitates the targeting of HIV integration by acting on chromatin topology. We show
Peter Dy et al.
Nucleic acids research, 36(9), 3101-3117 (2008-04-12)
The group C of Sry-related high-mobility group (HMG) box (Sox) transcription factors has three members in most vertebrates: Sox4, Sox11 and Sox12. Sox4 and Sox11 have key roles in cardiac, neuronal and other major developmental processes, but their molecular roles
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