B2904
Bluo-Gal
reagent for selection of recombinant bacterial clones
Sinónimos:
5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
Acerca de este artículo
Fórmula empírica (notación de Hill):
C14H16BrNO6
Número CAS:
Peso molecular:
374.18
UNSPSC Code:
12352200
NACRES:
NA.85
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1550140
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlegrade
Molecular Biology
Quality Level
sterility
non-sterile
assay
≥98% (HPLC)
form
powder
technique(s)
nucleic acid detection: suitable
solubility
DMF: soluble
suitability
suitable for substrate for β-galactosidase
storage temp.
−20°C
SMILES string
OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
InChI key
LINMATFDVHBYOS-MBJXGIAVSA-N
General description
Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Application
Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.
Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.
Biochem/physiol Actions
When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Leslie M Stone et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(11), 4522-4529 (2002-06-01)
Taste buds are a heterogeneous population of cells exhibiting diverse morphological and biochemical characteristics. Because taste buds arise from multiple progenitors, the different types of taste cells may represent distinct lineages. The present study was undertaken to determine the following:
Kyoji Horie et al.
Molecular and cellular biology, 23(24), 9189-9207 (2003-12-03)
The use of mutant mice plays a pivotal role in determining the function of genes, and the recently reported germ line transposition of the Sleeping Beauty (SB) transposon would provide a novel system to facilitate this approach. In this study
H T Yuan et al.
Journal of the American Society of Nephrology : JASN, 11(6), 1055-1066 (2000-05-23)
Angiopoietin-1 (Ang-1) stimulates endothelial and vascular network differentiation through the Tie-2 receptor tyrosine kinase, while Ang-2 modulates this activation in embryo and tumor growth. The nephrogenic pattern of Ang-2 was documented in a mouse strain that expresses the LacZ reporter