C2780
Condroitinasa AC from Flavobacterium heparinum
lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
Sinónimos:
Condroitina AC liasa
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
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Permítanos ayudarleNombre del producto
Condroitinasa AC from Flavobacterium heparinum, lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
conjugate
(Glucosaminoglycan)
form
lyophilized powder
specific activity
0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
composition
Protein, ~15% Lowry
solubility
0.02 M phosphate buffer: soluble (pH 7.0)
foreign activity
Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount
storage temp.
−20°C
Quality Level
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Other Notes
Véase más información sobre enzimas para análisis de carbohidratos complejos en www.sigma-aldrich.com/enzymeexplorer
Contains potassium phosphate buffer salts and BSA as stabilizer.
One unit will cause a ΔA232 of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).
Application
Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.
Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.
Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides
Biochem/physiol Actions
Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).
Preparation Note
Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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