C7617
Anti-Calnexin antibody, Mouse monoclonal
clone TO-5, purified from hybridoma cell culture
Sinónimos:
Anti-CANX, Anti-CNX
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Conjugate:
unconjugated
Clone:
TO-5, monoclonal
Application:
ELISA (i), FACS, ICC, IHC, WB
Species reactivity:
human
Citations:
19
Technique(s):
flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 0.5-1.0 μg/mL using total cell extract of HeLa cells.
Uniprot accession no.:
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Permítanos ayudarlebiological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
TO-5, monoclonal
form
buffered aqueous solution
mol wt
antigen ~90 kDa
species reactivity
human
enhanced validation
independent
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concentration
~2 mg/mL
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 0.5-1.0 μg/mL using total cell extract of HeLa cells.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CANX(821)
Application
Anti-Calnexin antibody, Mouse monoclonal has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- flow cytometry
- immunocytochemistry
- immunohistochemistry
- immunofluorescence analysis
Biochem/physiol Actions
Mouse monoclonal clone TO-5 anti-Calnexin antibody recognizes human Calnexin, ~90 kDa.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-Calnexin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma TO-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Calnexin contains a long (461 amino acids) N-terminal Ca2+-binding domain extending into the lumen of the endoplasmic reticulum (ER), a short (22 amino acids) trans-membrane segment and an acidic cytosolic domain (96 amino acids).
Calnexin (p88, IP90) is a calcium-binding, type I integral membrane protein, localized primarily in the endoplasmic reticulum (ER). Calnexin binds newly synthesized glycoproteins and misfolded proteins and is believed to play a critical role in quality control processes during protein synthesis and folding. Calnexin acts as a lectin like chaperone that binds oligosaccharide residues of newly synthesized N-linked glycoproteins. The lectin specificity of calnexin and its soluble homologue calreticulin has been identified as high mannose oligosaccharides terminating in monoglucosyl residues linked through α1-3. Calnexin has been shown to be associated with several cell surface proteins, including MHC class I heavy chain, T-cell receptor (TCR), and B cell membrane immunoglobulin during translocation through the ER. It also forms complexes with other resident ER proteins involved in Ca2+-dependent retention of proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Calreticulin, a multi-process calcium-buffering chaperone of the endoplasmic reticulum
Michalak M, et al.
The Biochemical Journal, 417(3), 651-666 (2009)
Aleksandr Treyer et al.
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