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Merck

C7901

Chelex® 100 sodium form

50-100 mesh (dry)

Sinónimos:

Iminodiacetic Acid Resin

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About This Item

Número CAS:
11139-85-8
UNSPSC Code:
23151817
NACRES:
NA.56
MDL number:
MFCD00163980

Nombre del producto

Chelex® 100 sodium form, 50-100 mesh (dry)

form

beads

matrix

1% cross-linked polystyrene
 cross-linked polystyrene beads

matrix active group

iminodiacetic acid (sodium form)
paired iminodiacetate groups

matrix attachment

through methyl group to aromatic rings

particle size

50-100 mesh (dry)

pH

4-14

capacity

≥0.3 meq/mL binding capacity
 0.6 meq/g binding capacity (heavy metal ions)

Quality Level

200

Application

Chelex se utiliza en la cromatografía de afinidad, la cromatografía de proteínas, las resinas quelantes, las resinas de afinidad y los biorreactivos centrales.
La forma sódica de Chelex® 100 se ha utilizado:
  • para la extracción de ADN de muestras de abejas
  • para eliminar del extracto de humo de los cigarrillos (CSE) y estudiar los efectos de la expresión de la hepcidina en los pulmones murinos
  • para extraer ADN de cultivos bacterianos

Biochem/physiol Actions

Chelex® 100 es un copolímero de estireno-divinilbenceno que contiene iones de iminodiacetato emparejados, que actúan como grupos quelantes en la unión de iones metálicos polivalentes. Se considera una resina débilmente ácida. Chelex actúa como una resina quelante que se utiliza en la extracción del ADN de muestras forenses, como el cabello, las tarjetas con manchas de sangre seca y los hisopos bucales.

Other Notes

El producto es esterilizable en autoclave en su forma sódica Tiene una temperatura de funcionamiento máxima de 75 °C y no es soluble en agua.

Legal Information

Chelex is a registered trademark of Bio-Rad Laboratories, Inc.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000513092
0000513092
0000511968
0000511968
0000511958

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What solvent should I use to swell the Product C7901, Chelex 100 at the beginning?

    We recommend using a good grade of water (deionized or distilled).

  4. My Chelex 100 (Product C7901) in my column dried out.  Can I still use it?

    The resin may be rehydrated and used, as long as it remains white.  If the color of the re-swollen resin is yellow, do not use it.

  5. I want to use Product C7901, Chelex 100, to make a metal-free solution.  However, the water wash from the Chelex resin is very basic.  How can I fix this?

    A suspension of Chelex 100 (sodium form) in water typically has a pH of 10-11. This pH can be slowly lowered by extended water washing, or by using a buffer.  The sodium form at pH 6.3 can be obtained by rinsing with at least 4 bed volumes of 0.5 N sodium acetate buffer, followed by 5 bed volumes of water. This is a more common method for making metal-free distilled water.

  6. What is the particle size of the resin in Product C7901, Chelex 100?

    The particle size for this resin is 45-165 microns. The mean particle size is 90 microns.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Contenido relacionado

Product Information Sheet - C7901

Product Information Sheet

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