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Merck

C7902

Cloruro de calcio dihydrate

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

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About This Item

Fórmula empírica (notación de Hill):
CaCl2 · 2H2O
Número CAS:
10035-04-8
Peso molecular:
147.01
NACRES:
NA.71
PubChem Substance ID:
24892994
UNSPSC Code:
12352302
EC Number:
233-140-8
MDL number:
MFCD00149613
assay:
≥99.0%
form:
powder
solubility:
H2O: 294 mg/mL, clear, colorless

Nombre del producto

Cloruro de calcio dihydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

InChI key

LLSDKQJKOVVTOJ-UHFFFAOYSA-L

InChI

1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2

SMILES string

O.O.Cl[Ca]Cl

product line

BioReagent

assay

≥99.0%

form

powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

mp

176 °C (dec.) (lit.)

solubility

H2O: 294 mg/mL, clear, colorless

Quality Level

200

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Application

Calcium chloride dihydrate has been used:
  • in the formulation of media
  • in sperm collection and preparation
  • in cell culture and protoplast preparation

General description

Calcium chloride (CaCl2) is a commonly used reagent in biochemistry. It plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability. CaCl2 is used in the preparation and transformation of competent Escherichia coli (E. coli) and the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. CaCl2 is used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from E. coli. The crystallization of porcine pancreatic elastase in the presence of CaCl2 along with sodium citrate reveals the binding of calcium in the metal-binding site of the protein. The effect of CaCl2 on the total fluorescence in the polymerization of the tubulin-like filamenting temperature-sensitive mutant Z (FtsZ) division protein of E. coli has been studied by a multiwell fluorescent assay.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
0000503971
0000503971
0000490318
0000490318
0000474932

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The effects of L-ergothioneine and L-ascorbic acid on the in vitro maturation (IVM) and embryonic development (IVC) of sheep oocytes
PANCARCI S M, et al.
Kafkas U?niversitesi veteriner faku?ltesi dergisi, 16(5) (2010)
Hemolymph analysis and evaluation of newly formulated media for culture of shrimp cells (Penaeus stylirostris)
Shimizu C, et al.
In Vitro Cellular & Developmental Biology. Animal, 37(6), 322-329 (2001)
Biomolecule delivery into canola protoplasts by centrifuging cells with microbubbles
He C, et al.
Febs Letters, 587(3), 285-290 (2013)
Marco Wallenborn et al.
PloS one, 15(7), e0234986-e0234986 (2020-07-08)
Glioblastoma is a common, malignant brain tumor whose disease incidence increases with age. Glioblastoma stem-like cells (GSCs) are thought to contribute to cancer therapy resistance and to be responsible for tumor initiation, maintenance, and recurrence. This study utilizes both SNP
Aimee K Murray et al.
Environmental health perspectives, 128(10), 107007-107007 (2020-10-22)
Antimicrobial resistance (AMR) is one of the most significant health threats to society. A growing body of research demonstrates selection for AMR likely occurs at environmental concentrations of antibiotics. However, no standardized experimental approaches for determining selective concentrations of antimicrobials
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Product Information Sheet - C7902

Product Information Sheet

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