Saltar al contenido
Merck

F4021

Follicle Stimulating Hormone from human pituitary

~7,000 IU/mg (powder)

Sinónimos:

FSH, Follitropin

Iniciar sesión para ver los precios por organización y contrato.

Seleccione un Tamaño

Todas las fotos(1)

About This Item

Número CAS:
9002-68-0
UNSPSC Code:
51111800
eCl@ss:
42020504
NACRES:
NA.32
MDL number:
MFCD00131099
biological source:
human pituitary glands
form:
powder

Nombre del producto

Follicle Stimulating Hormone from human pituitary, ~7,000 IU/mg (powder)

biological source

human pituitary glands

sterility

non-sterile

form

powder

specific activity

~7,000 IU/mg (powder)

mol wt

35 kDa

technique(s)

cell culture | mammalian: suitable

impurities

HBsAg, HCV and HIV-1/HIV-2, tested negative

pH

7.4

solubility

water: ~50 g/L

UniProt accession no.

P01225
P23945

shipped in

ambient

storage temp.

−20°C

Quality Level

200

Gene Information

human ... FSHB(2488), FSHR(2492)

Categorías relacionadas

Application

Follicle stimulating hormone (FSH) from human pituitary has been used for-
  • in vitro luteinization of Macaque granulosa cells
  • luteal cell proliferation assay
  • culturing of preantral follicle cells obtained from mice
  • in vitro maturation of immature cumulus-oocyte complexes (COCs) obtained from mice and
  • culture of RAW264.7 cell line

Biochem/physiol Actions

FSH (follicle stimulating hormone) in involved in the growth of ovarian follicles and steroidogenesis of ovaries. FSHR (follicle-stimulating hormone receptor) interacts with FSH in the ovaries and determines the responsiveness to this hormone. Depressed levels of FSHR result in poor responsiveness and influence folliculogenesis. In the Caucasian population, certain polymorphisms in the FSHR gene are more commonly found in patients with polycystic ovary syndrome (PCOS). In the male fetuses and neonates, it facilitates the proliferation of Sertoli cells. In pubescent males, it stimulates spermatogonia to undergo mitosis.FSH upon binding to its receptor FSH-R positively regulates G-protein coupled receptors/cyclic adenosine monophosphate (cAMP)/protein kinase A pathways to cascade intracellular signals.
The α-chain is not active; biological specificity is attributed to the β-chain. Induces maturation of Graafian follicles of the ovary; promotes development of germinal cells in males; activates cytosolic tyrosine kinase.

General description

The α chain of FSH has 89 amino acids and the β chain has 115 amino acids. The α chain is not active. FSH belongs to glycoprotein hormone family. It is secreted by the gonadotropic cells of pituitary gland.
Research area: Cell signaling

Other Notes

Two-chain glycoprotein hormone

Physical form

Contains 0.1 mg of 0.05M PBS buffer (pH 7.4)

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
MKDB0460
MKDB0461
MKDB0458
MKDB0455
MKCZ6107

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. For Product F4021, Follicle Stimulating Hormone, do you have information on reconstitution, solution storage and potency?

    Product F4021-1VL is packaged as 50 units/vial. Each vial contains roughly 35-36 milligrams solid. Our solubility testing is done at 50 mg/mL, which yields a slightly hazy solution. It may be more convenient to use units/mL for your experiment as it will be more precise. If you decide to try to weigh out the material, please realize that small quantities can be difficult to weigh accurately.For Product F4021, FSH from human pituitary, we recommend solution storage for up to one month at 2-8°C. Unfortunately, we do not have any Sigma data regarding the stability of the porcine hormone.In the absence of information, we would recommend that solutions of Product F4021 not be frozen. If you do decide to store the product at -20°C we would suggest storing it in single use aliquots to avoid numerous freeze/thaw cycles.For the human FSH product we list an approximate activity of 7000 International Units/milligram. This should provide you with a starting point for a conversion calculation. For the porcine product we do not provide a conversion for units to mass. We provide 50 Armour units per vial. The unit definitions are also slightly different. International units are based on an international standard (IRP-68/140) while Armour units are based on Armour FSH standard G-94. According to the literature, 1 Armour unit can contain 9.4-14.2 international units.

  6. Do you have information on the solution stability of Follicle Stimulating Hormone (FSH) from pituitary?

    For information on the solution stability of FSH (and much more), refer to Chem. Soc. Rev., 2(4), 355-395 (1973).From page 369:  "The stabilities of the most highly purified preparations of certain hormonal glycoproteins have presented some problems, e.g. follicle-stimulating hormone preparations are often found to be stable in frozen solution but lose activity dramatically on concentration and freeze drying." Based on that information, we would recommend that if you need to freeze solutions of FSH, you should flash-freeze the solution (using either liquid nitrogen or a dry ice-alcohol bath), and store the solution in the ultra-cold (-70 or -80°C) freezer. Since FSH does have some solution stability concerns, simply placing the solution in a -20°C freezer without first flash-freezing may reduce the activity.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Karenne N Fru et al.
Biology of reproduction, 75(4), 568-574 (2006-07-14)
Ovulation and luteal formation in primates are associated with the sustained synthesis of progesterone. The observed high intrafollicular concentrations of progesterone during the periovulatory interval raise the possibility that this steroid serves as a precursor for mineralocorticoids. The aim of
Y Figenschau et al.
Human reproduction (Oxford, England), 12(3), 523-531 (1997-03-01)
A simplified method for the preparation and long-term cultivation of granulosa-luteal cells in serum-free medium is described. The cells were harvested from women undergoing in-vitro fertilization, enriched by sedimentation and dissociated by enzymatic treatment. We demonstrated, by introducing a synthetic
J Wu et al.
Biology of reproduction, 63(1), 320-327 (2000-06-22)
Although it is known that LH receptors are present from the time of thecal differentiation, the role of LH during early follicle development is not yet clear. The effect of LH on preantral follicle development has therefore been investigated in
Alfredo Ulloa-Aguirre et al.
Endocrinology, 159(8), 3020-3035 (2018-07-10)
FSH is synthesized in the pituitary by gonadotrope cells. By binding to and interacting with its cognate receptor [FSH receptor (FSHR)] in the gonads, this gonadotropin plays a key role in the control of gonadal function and reproduction. Upon activation
Marina Grigorova et al.
The Journal of clinical endocrinology and metabolism, 96(9), E1534-E1541 (2011-07-08)
The detailed role of FSH in contributing to male testicular function and fertility has been debated. We have previously identified the association between the T-allele of the FSHB promoter polymorphism (rs10835638; G/T, -211 bp from the mRNA start) and significantly
Ver todos los artículos relacionados

Contenido relacionado

Product Information Sheet - F4021

Product Information Sheet

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico