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Merck

G1875

β-Galactosidase from bovine liver

Grade III, lyophilized powder, ≥0.15 units/mg protein

Sinónimos:

β-D-Galactoside galactohydrolase

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About This Item

Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
Número CE:

Nombre del producto

β-Galactosidase from bovine liver, Grade III, lyophilized powder, ≥0.15 units/mg protein

biological source

bovine liver

type

Grade III

form

lyophilized powder

specific activity

≥0.15 units/mg protein

composition

Protein, ≥40%

shipped in

wet ice

storage temp.

−20°C

Quality Level

Application

β-galactosidase was used in the production of a stabilized, single reagent for alcohol analysis.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Other Notes

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Stabilization of Analytical Enzymes Using a Novel Polymer-Carbohydrate System and the Production of a Stabilized, Single Reagent for Alcohol Analysis
Timothy D. Gibson, John Higgins, John R. Woodward
Analyst, 117, 1293-1297 (1992)
Alen Sevšek et al.
ChemMedChem, 12(7), 483-486 (2017-03-23)
A series of lipidated guanidino and urea derivatives of 1,5-dideoxy-1,5-imino-d-xylitol were prepared from d-xylose using a concise synthetic protocol. Inhibition assays with a panel of glycosidases revealed that the guanidino analogues display potent inhibition against human recombinant β-glucocerebrosidase with IC50
D H Juers et al.
Protein science : a publication of the Protein Society, 8(1), 122-136 (1999-04-21)
Beta-galactosidase (lacZ) from Escherichia coli is a 464 kDa homotetramer. Each subunit consists of five domains, the third being an alpha/beta barrel that contains most of the active site residues. A comparison is made between each of the domains and
Staci Rakowiecki et al.
Development (Cambridge, England), 140(8), 1730-1739 (2013-03-15)
The morphogenetic program that shapes the three semicircular canals (SSCs) must be executed with extreme precision to satisfy their complex vestibular function. The SSCs emerge from epithelial outgrowths of the dorsal otocyst, the central regions of which fuse and resorb
Yiquan Zhang et al.
Future microbiology, 8(2), 271-280 (2013-02-05)
To explore the transcriptional regulation of the psaEF and psaABC loci by the RovA and PhoP regulators in Yersinia pestis. Primer extension, LacZ fusion, gel mobility shift and DNase I footprinting assays were conducted in combination for this gene regulation

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