G3153
β-Galactosidasa from Escherichia coli
lyophilized powder, ≥500 units/mg protein
Sinónimos:
β-D-galactósido galactohidrolasa, Lactasa
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About This Item
Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
Nombre del producto
β-Galactosidasa from Escherichia coli, lyophilized powder, ≥500 units/mg protein
recombinant
expressed in E. coli
form
lyophilized powder
specific activity
≥500 units/mg protein
mol wt
465 kDa
storage temp.
−20°C
Quality Level
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Categorías relacionadas
Biochem/physiol Actions
βLa β-galactosidasa hidroliza la lactosa en sus componentes monosacáridos, glucosa y galactosa. También cataliza la transglucosilación de la glucosa en alolactosa, el inductor de la β-galactosidasa, en un bucle de retroalimentación.
β-galactosidase is considered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure and target gene expression.
General description
Peso molecular del tetrámero 465 kDa (subunidades de 116,3 kDa cada una)
β-galactosidase is a 465 kDa tetrameric protein. It is coded by the LacZ gene.
Application
β-Galactosidase from Escherichia coli has been used to produce biocatalytic Ca-alginate beads on a B-390 encapsulator (BUCHI). It has been used to prepare the source droplet solution and also as the cargo for Pep-1 (KETWWETWWTEWSQPKKKRKV-cysteamide).
β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration.
Other Notes
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactopyranoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
Packaging
Package size based on protein content
Physical form
Stabilized with phosphate buffer and sucrose
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Peptide-Mediated Membrane Transport of Macromolecular Cargo Driven by Membrane Asymmetry
Li X, et al.
Analytical Chemistry, 89(22), 12369-12374 (2017)
M J O'Sullivan et al.
Annals of clinical biochemistry, 16(5), 221-240 (1979-09-01)
In the last few years, the use of enzyme labels in immunoassays has been investigated. The aim of this review is to evaluate critically the role of such labels in clinical biochemistry. Special attention has been given to the problems
Structure of beta-galactosidase at 3.2-AA resolution obtained by cryo-electron microscopy
Bartesaghi A, et al.
Proceedings of the National Academy of Sciences of the USA, 111(32), 11709-11709 (2014)
Samuel Berhanu et al.
Journal of basic microbiology, 62(6), 669-688 (2022-03-16)
pUC18 and pUC19 are well-known high copy-number plasmid vectors routinely used for DNA cloning purposes. We show here that, in Escherichia coli transformed by native pUC18, the α-complementation of β-galactosidase (i.e., mediated by the peptide LacZα18) is intrinsically weak and
Joaquin Seras-Franzoso et al.
Applied and environmental microbiology, 78(7), 2376-2385 (2012-01-31)
Escherichia coli β-galactosidase is probably the most widely used reporter enzyme in molecular biology, cell biology, and biotechnology because of the easy detection of its activity. Its large size and tetrameric structure make this bacterial protein an interesting model for
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