H3784

HEPES sodium salt

Name: HEPES sodium salt
Brand: SIGMA

BioPerformance Certified, suitable for cell culture, ≥99.0%

Sinónimos:

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

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About This Item

Fórmula empírica (notación de Hill):

C₈H₁₇N₂NaO₄S

Número CAS:

75277-39-3

Peso molecular:

260.29

NACRES:

NA.25

PubChem Substance ID:

24895595

UNSPSC Code:

12161700

EC Number:

278-169-7

MDL number:

MFCD00036463

Nombre del producto

HEPES sodium salt, BioPerformance Certified, suitable for cell culture, ≥99.0%

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

grade

BioPerformance Certified

assay

≥99.0%

form

powder

storage condition

dry at room temperature

technique(s)

activity assay: suitable
cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

color

white

pH

10-12 (1 M in water)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 0.1 M, clear, colorless

density

1.19 g/cm³ at 20—25 °C
1.21 g/cm³ at 20—25 °C

absorption

≤0.02 at 280 in H₂O at 0.1 M
≤0.04 at 260 in H₂O at 0.1 M

suitability

suitable for component for culture media
suitable for molecular biology

application(s)

cell analysis
diagnostic assay manufacturing
general analytical
life science and biopharma

foreign activity

DNase, RNase, NICKase, protease, none detected

Quality Level

400

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Categorías relacionadas

Biochemicals

Biological Buffers

Chemistry & Biochemicals

High-Purity Biochemicals and Reagents

Application

HEPES sodium salt has been used:

to supplement the HSOF medium
as a buffer component of HCZB medium in egg and embryo culture
as a buffer component in cell culture

Features and Benefits

High-purity product for biochemical and biological research
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
Suitable for Cell culture
Tested for Endotoxins and Total Aerobic Microbial Count
Free from DNase, NICKase, RNase, Endonuclease, Exonuclease and Protease

General description

HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer with a remarkable pH buffering capacity between 6.8 and 8.2 (pKa of 7.48 at 25°C). Widely recognized for its versatility, HEPES is extensively employed in cell biology, biochemical, and biological research. Notably, its neutrality towards metal ions makes it a preferred choice for solutions with metal ions, acting as a "non-coordinating buffer."

In cell culture, HEPES excels in maintaining stable physiological pH even amidst fluctuating carbon dioxide concentrations, outperforming bicarbonate buffers (NaHCO₃). Moreover, HEPES showcases valuable properties in various biological and biochemical processes, such as isoelectric focusing for protein separation and minimal interference in DNA-restriction enzyme reactions, making it indispensable across diverse research applications, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility, exceptional buffering capacity, and minimal interaction with molecules establish HEPES as a key tool in various research domains.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Physical form

Lyophilized, spray dried, or crystallized material.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue"

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Contenido relacionado

Product Information Sheet - H3784

Product Information Sheet

HEPES Sodium Salt Specification Comparison

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