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Merck

H4522

Suero humano

from human male AB plasma, USA origin, sterile-filtered

Sinónimos:

plasma humano, suero humano, sueros humanos

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:
MFCD00165829
biological source:
human male plasma (Type AB)
origin:
USA origin
sterility:
sterile-filtered
shipped in:
dry ice

Nombre del producto

Suero humano, from human male AB plasma, USA origin, sterile-filtered

biological source

human male plasma (Type AB)

sterility

sterile-filtered

composition

hemoglobin, ≤30 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

Quality Level

300

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Analysis Note

Analizada la presencia de endotoxinas

Application

El suero AB humano se utiliza en aplicaciones de ingeniería genética, trasplantes y terapia celular para la expansión de células madre mesenquimatosas (MSC) del tejido adiposo o las células del estroma mesenquimatoso de la médula ósea humana; para la generación y el trasplante de injertos normalizados de células madre del epitelio del limbo esclerocorneal; para la expansión ex vivo de células NK a partir de sangre periférica en medio de expansión de células madre hematopoyéticas; y para actualización de la tecnología de cultivo de islote humano pretrasplante.
El suero humano se ha utilizado como componente de la disolución salina tamponada con fosfato con detergente Tween® (PBST) en el enzimoinmunoanálisis de adsorción (ELISA) de doble captura, como muestra biológica simulada para evaluar el rendimiento del ELISA en papel con muestras biológicas complejas, como diluyente para crear una dilución en serie de la muestra basal para la generación de la curva de calibración para NISTmAb enriquecido en suero, así como para la muestra de paciente de mieloma múltiple (MM) MM2232

Biochem/physiol Actions

El suero AB humano se obtiene normalmente permitiendo la coagulación natural de sangre completa, lo que se conoce como suero «fuera del coágulo» (OTC). Se prefiere el suero humano obtenido de sangre completa coagulada, ya que en general contiene concentraciones mayores, y una gama más diversa, de factores de crecimiento para un crecimiento celular óptimo. El suero humano procedente de donantes de sangre de tipo AB carece de anticuerpos específicos para los antígenos de las sangres de tipo A y B. Esto lo convierte en un suero donante universal que es adecuado para varios tipos de cultivos celulares, entre ellas las líneas de células madre humanas.

Disclaimer

Se analizó cada donante para la hepatitis B y C y se observó que no eran reactivos , y tampoco lo eran para los anticuerpos del virus de la inmunodeficiencia humana (VIH) mediante ELISA. No obstante, los productos de origen humano deben considerarse potencialmente infecciosos y manejarse en consecuencia.
SÓLO PARA INVESTIGACIÓN. Este producto está regulado en Francia cuando está destinado a ser utilizado con fines científicos, como para actividades de importación y exportación (artículo L 1211-1, apartado 2, del Código de Salud Pública). El comprador (es decir, el usuario final) está obligado a obtener una autorización de importación del Ministerio de Investigación de Francia referida al artículo L1245-5-1 II del Código de Salud Pública. Al pedir este producto, usted confirma que ha obtenido la autorización de importación adecuada.

Other Notes

No es inusual que nuestros productos séricos humanos estén turbios. Este producto procede de la combinación de sueros y la turbidez indica que hay lípidos en el suero. No se dispone de productos séricos humanos transparentes, carentes de lípidos.

Packaging

El embalaje puede variar para diferentes lotes debido a la escasez de las botellas utilizadas para embalar este producto. La presencia o falta de envoltura retráctil alrededor de la tapa de la botella no afecta a la integridad/esterilidad del producto.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000399478
0000399478
0000399472
0000399472
0000399518

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. There is a precipitate in my serum.  Is my serum contaminated?

    Precipitates in serum can often appear as large round bodies, even as doublets per microscopic exam. This can be mistaken as a yeast contamination. Brownian motion may be mistaken for motility. These are lipid aggregates that will be readily dissolved when exposed to lipase. Also, these particles will not gram stain or show any growth in culture on aerobic, anaerobic, or fungal media and culture conditions. The performance of the material is not effected. Centrifugation or filtration of small volumes will remove these particles.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. Is Product H4522, Human serum, Heat Inactivated (HI)?

    This serum is not heat inactivated. If heat inactivated serum is needed, the serum can be heated to 56 °C for 30 minutes. Heating inactivates the complement cascade. Active complement can participate in cytolytic events, leading to contraction of smooth muscle, histamine release from mast cells and platelets, and activation of lymphocytes and macrophages.

  5. How is Product H4522, Human serum, prepared?

    Blood is collected and has citrate added as an anticoagulant to inhibit clotting.  The blood is then centrifuged and separated into cells and plasma.  The plasma is separated from the cells, and calcium is added to the plasma to activate the clotting cascade.  The preparation is then centrifuged again, and the serum is the liquid product remaining.  This process yields a serum low in growth factors, as it does not contain those factors released from the cells upon clotting.

  6. Product H4522, Human serum, is cloudy. What causes the cloudiness?

    It is not unusual for our human serum products to be cloudy. This product is from pooled serum and cloudiness indicates that lipids are present in the serum. No clear, lipid-free human serum products are available.

  7. Why do we specify male AB in the description of Product H4522, Human serum?

    Male blood is used in serum products, where specified, because female serum can have more antibodies resulting from pregnancy.  Type AB serum is used as there are no anti-A and/or anti-B antibodies that are found in other blood types.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. What is the difference between human serum from AB plasma and  human serum from AB clotted whole blood?

    Product H6914 is prepared from whole blood that is allowed to clot, centrifuged and the serum removed. There is no additional processing required.Product H4522 is prepared from anticoagulated (sodium citrate) whole blood. This is centrifuged to pellet the cells (RBC, WBC, some platelets) and the plasma is removed. This plasma is then treated with calcium to activate the clotting cascade. The preparation is centrifuged again and the supernatant is removed. This product can also be called plasma derived serum.Product H6914 will contain more growth factors since it is allowed to spontaneously clot and there will be the release of growth factors from many of the cells (WBC, platelets) present.  The cells are removed before the plasma is clotted for Product H4522, so this plasma will have fewer growth factors present (more similar to what is circulating). Unless testing for growth factors, most researchers are looking for Product H6914.We offer both products heat inactivated.Product H5667 Human Serum Heat Inactivated, from male AB clotted whole bloodProduct H3667 Human Serum Heat Inactivated, from human male AB plasma

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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