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Merck

L3772

Leptin from mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Sinónimos:

OB

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Número CAS:
181030-10-4
UNSPSC Code:
51111800
NACRES:
NA.32
MDL number:
MFCD01094206
Biological source:
mouse
Form:
lyophilized powder
Assay:
≥98% (SDS-PAGE)

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biological source

mouse

Quality Level

200

recombinant

expressed in E. coli

sterility

non-sterile

assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

≤1.0 ng/mL ED50

mol wt

predicted mol wt ~16 kDa

storage condition

OK to freeze

technique(s)

cell based assay: suitable

impurities

≤1.0 EU/μg endotoxin

UniProt accession no.

P41160

shipped in

ambient

storage temp.

−20°C

SMILES string

CC(C)CC(C(=NC(CCC(=N)O)C(=NCC(=NC(CO)C(=NC(CC(C)C)C(=NC(CCC(=N)O)C(=NC(CC(=O)O)C(=NC(CCSC)C(=NC(CC(C)C)C(=NC(CC1=CNC2=CC=CC=C21)C(=NC(CCC(=N)O)C(=NC(CC(C)C)C(=NC(CC(=O)O)C(=NC(CC(C)C)C(=NC(CO)C(=O)N3CCCC3C(=NCC(=NC(CS)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N

InChI

1S/C87H138N22O28S2/c1-41(2)27-48(88)72(121)97-50(18-21-65(89)112)73(122)93-36-68(115)95-61(38-110)84(133)104-54(28-42(3)4)77(126)98-52(20-23-67(91)114)75(124)106-59(33-70(117)118)82(131)100-53(24-26-139-11)76(125)102-55(29-43(5)6)78(127)105-58(32-46-35-92-49-16-13-12-15-47(46)49)81(130)99-51(19-22-66(90)113)74(123)101-56(30-44(7)8)79(128)107-60(34-71(119)120)83(132)103-57(31-45(9)10)80(129)108-62(39-111)86(135)109-25-14-17-64(109)85(134)94-37-69(116)96-63(40-138)87(136)137/h12-13,15-16,35,41-45,48,50-64,92,110-111,138H,14,17-34,36-40,88H2,1-11H3,(H2,89,112)(H2,90,113)(H2,91,114)(H,93,122)(H,94,134)(H,95,115)(H,96,116)(H,97,121)(H,98,126)(H,99,130)(H,100,131)(H,101,123)(H,102,125)(H,103,132)(H,104,133)(H,105,127)(H,106,124)(H,107,128)(H,108,129)(H,117,118)(H,119,120)(H,136,137)/t48-,50-,51-,52-,53-,54-,55-,56-,57-,58-,59-,60-,61-,62-,63-,64-/m0/s1

InChI key

NRYBAZVQPHGZNS-ZSOCWYAHSA-N

Gene Information

mouse ... Lep(16846)

Categorías relacionadas

General description

Research area: Cell signaling

Leptin (Ob) is a non-glycosylated polypeptide hormone. It is produced primarily in adipocytes and the placenta. The structure of leptin closely resembles members of the long-chain helical cytokine family. It is composed of four antiparallel α-helices interconnected by two long crossover links and one short loop. These structural elements are arranged in a left-handed helical bundle formation, resulting in a unique two-layer packing configuration.

Application

Leptin from mouse has been used:
  • to check the effect of leptin on ovulation for treating infertility in obese mice
  • to check the effect of leptin on MGARP (mitochondria-localized glutamic acid-rich protein) expression in testis and follicular granular cells of mice
  • to study the morphometrical changes in mouse testicular tissue upon long term exposure to leptin

Biochem/physiol Actions

Hormone produced primarily in adipocytes; primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight.
Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo.
Human and mouse leptin share ~84% sequence identity.
Leptin, an essential cytokine, serves as a signaling molecule in the reproductive system. It modulates placental functions through autocrine or paracrine signaling. Particularly in the early stages of pregnancy, leptin plays a vital role in modulating key processes including cell proliferation, protein synthesis, invasion, and apoptosis within placental cells. Deficiency of leptin may lead to the development of severe obesity and abnormalities in hematopoiesis, immunity, angiogenesis, bone formation, blood pressure regulation and reproduction. Absence of leptin in mice causes severe obesity and hypothermia at temperature below thermoneutrality. It also controls feeding behavior. Leptin signaling is crucial for the bone formation.

Physical form

Lyophilized from a 0.2 μm filtered solution with 0.0045 mM NaHCO3

Analysis Note

The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.

wgk

WGK 3

Clase de almacenamiento

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000469971
0000469971
0000264063
0000264063
049M4787V

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Leptin action in normal and pathological pregnancies
Perez-Perez P, et al.
Journal of Cellular and Molecular Medicine, 22(2), 716-727 (2018)
Intracellular signalling pathways activated by leptin
Fruhbeck G
The Biochemical Journal, 393(Pt 1), 7-20 (2006)
Mohammad-Reza Esmaili-Nejad et al.
Iranian journal of basic medical sciences, 18(12), 1176-1182 (2016-02-16)
Leptin is a novel and interesting hormone for anyone trying to lose weight, but its effects on male gonad structure in longitudinal study is unknown. The present study was designed to explore morphometrical changes of mouse testicular tissue after long-term
Yan Gao et al.
The Journal of clinical investigation, 125(9), 3681-3691 (2015-08-25)
Dietary iron supplementation is associated with increased appetite. Here, we investigated the effect of iron on the hormone leptin, which regulates food intake and energy homeostasis. Serum ferritin was negatively associated with serum leptin in a cohort of patients with
Takeru Sakai et al.
American journal of physiology. Endocrinology and metabolism, 307(8), E712-E719 (2014-08-28)
Leptin may reduce pancreatic lipid deposition, which increases with progression of obesity and can impair β-cell function. The insulinotropic effect of glucagon-like peptide-1 (GLP-1) and the efficacy of GLP-1 receptor agonist are reduced associated with impaired β-cell function. In this
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Data Sheet - L3772

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