L7647
Concanavalin A from Canavalia ensiformis (Jack bean)
Type VI, lyophilized powder
Sinónimos:
Con A
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Permítanos ayudarleNombre del producto
Concanavalin A from Canavalia ensiformis (Jack bean), Type VI, lyophilized powder
biological source
Canavalia ensiformis
type
Type VI
form
lyophilized powder
potency
≤20 μg per mL agglutination activity
impurities
Carbohydrate, essentially free
salt, essentially free
solubility
water: 1 % (w/v)
storage temp.
−20°C
Quality Level
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Analysis Note
Agglutination activity: Determined from serial dilutions of a 1 mg/mL solution (this activity is the lowest conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C). Requires Type A Human Blood.
Application
Concanavalin A from Canavalia ensiformis (Jack bean) has been used for developing zwitterion-immobilized molecularly imprinted polymers (ziMIPs) for protein crystallization. It has been also used in the preparation of protein loaded microgels to prevent enzymatic degradation of microgels for monitoring by confocal laser scanning microscope (CLSM).
Biochem/physiol Actions
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Preparation Note
Highly purified by specific affinity chromatography.
signalword
Danger
hcodes
Hazard Classifications
Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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