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Merck

M2279

Medio esencial mínimo Eagle

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

MEM

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UNSPSC Code:
12352207
NACRES:
NA.75
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Nombre del producto

Medio esencial mínimo Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

General description

Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. It includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for the cultivation of a wide variety of cells grown in monolayers and attached cell lines, such as fibroblasts, in the presence of fetal bovine serum (FBS), calf, or horse sera.

Application

Minimum Essential Medium Eagle has been used to culture the:
  • explants of aortic valve leaflets
  • human liver cancer cell line (HepG2) cells
  • human fetal brain progenitor-derived astrocyte cells (PDA)
  • breast cancer cell line (MCF-7)
  • human glioblastoma cell line (T98G)

Preparation Note

Supplement with 0.292 g/L L-glutamine.


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Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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